Stilbene Glycoside From Polygonum Multiflorum Inhibite UVA-induced Oxidative Damage To HaCaT Keratinocytes

Objective To investigate the stilbene glucoside's protective ability to UVA-induced oxidative damage to HaCaT keratinocytes. Methods Stilbene glycoside was isolated from Polygonum multiflorum. The UVA radiant intensity was 18J/cm2, and human keratinocytes HaCaT were used as an experimental in vitro model. To test the cell viability by MTT; Enzyme biochemical method determined the cytoplasmic SOD, GSH-px activity and MDA levels. The Human Oxidative Stress and Antioxidant Defense RT2 ProfilerTM PCR Array was used to detect the related intracellular gene's mRNA changes, and its results were validated by Real-time PCR. Results Under 18J/cm2 UVA radiation, within the scope of a given concentration of stilbene glucoside can dose-dependently increase SOD, GSH-px activity and decrease MDA level in the cytoplasm; gene chip detected that stilbene glucoside can significantly change some oxidative and antioxidative genes expression, and PCR Array's results were validated conformably by Real-time PCR. Conclusion Stilbene glycoside have some protective effect on UVA-induced oxidative injury to HaCaT keratinocytes. Its mechanism may be related to the elimination of free radicals, the increasing of SOD and GSH levels, changs in some related genes expression.