The Effect Of Astacene On Mice Skeletal Development And Energy Metabolism

Astacene, as a kind of non-vitamin A carotenoid, mainly resist in aquatic animal and alga. It has strong anti-oxidant, and important effects in many directions such as freeing radical scavenging, clear ROS, anticancer and skeletal power enhancement.In this study, mice skeletal muscle and primary cμLtural skeletal cell were used as the object, which were treated with astacene of different concentrations. The body weight of mice as well as feed intake varies were determined; skeletal muscle histological section and the ability of antioxidase were detected; by using semi-quantitative RT-PCR technique, the mRNA levels of Myod, Myf5, LXRα, UCP3, PPARγ, FAS and HSL genes in skeletal muscle tissues and skeletal cells were determined after different treatment periods; the effects of astacene on skeletal cell proliferation, differentiation and energy metabolism trough ERK signal pathway were analyzed; the effect of astacene on mice skeletal muscle development and energy metabolism were investigated. The resμLts were as following:1. Feed intake of mice was increased and body weight gain per day was decreased after treatment with 3.07g/Kg astacene (P<0.05), 30 days later, the length and density of skeletal muscle fibers were both increased, and the mRNA levels of Myod, LXRαand HSL were increased (P<0.05), while the mRNA levels of UCP3, PPARγand FAS were decreased (P<0.05); the expression of Myf5 gene in skeletal muscle were decreased after treatment with 0.768g/Kg astacene for 30 days. It can be seen that astacene can conduce to the skeletal muscle development and energy metabolism, decrease the inter-muscμLar fat mass in skeletal, and enhance oxygenolysis process, which might be the reason of loss of mice body weight.2.5μmol/L astacene can continuously enhance skeletal cell proliferation (P<0.01); the expressions of Myod gene were both increased when after 24h and 72h treatment; when skeletal cell were treated with 15μmol/L astacene, the proliferation of skeletal cells were increased (P<0.05), the expressions of LXRα, Myf5 and Myod genes were increased after 72h (P<0.05), and the expression of UCP3 was significantly decreased (P<0.01). It can be demonstrated that astacene at lower concentration can increase the skeletal cell proliferation; the higher concentration can enhance the skeletal differentiation, and also can promote the energy use of skeletal cells.3. PD98059, as the inhibitor of ERK signal pathway, can depress the proliferation of skeletal cell when at the concentration of 20μmol/L (P>0.05), the expressions of Myod and LXRα were decreased (P<0.05), and Myf5 and UCP3 were increased (P<0.05). the expressions of Myod and LXRαwere increased when treated with astacene combine with the inhibitor. It is demonstrated that astanece can activate the ERK signal way, and also has a certain positive role on skeletal cell proliferation, differentiation and energy usage.4. The activity of SOD and CAT in skeletal muscle tissue were remarkablely increased after 10 days'treatment with 0.768 g/Kg astacene, and the MDA content and GSH-PX activity were decreased (P<0.05). After treating 30 days, the activity of CAT increased significantly (P<0.01), the activity of GSH-PX was decreased (P<0.05). The astacene at concentration of 3.071 g/Kg can increase the activity of SOD, CAT and GSH-PX in skeletal muscle tissue (P<0.05), and decrease the content of MDA (P<0.01). It demonstrated that astacene can clean the oxyradical in skeletal tissue efficiently, play the protective function in skeletal muscle tissue.