Studies On Antidepressant Constituents Of Radix Polygalae And Preparative Separation Of Yuan Zhi Oligosaccharide Esters And Myricitrin Using HSCCC

Studies on antidepressant constituents of Radix Polygalae and preparative separation of yuan zhi oligosaccharide esters and myricitrin using HSCCCSeven compounds were isolated from the part of Polygala tenuifolia with antidepressant effect by various column chromatographies. Their structures were elucidated on the basis of spectral data (including IR, UV,1HNMR,13CNMR, EI-MS etc.). There are five oligosaccharide esters and two xanthones. They are 3,6'-disinapoylsucrose, tenuifoliside A, tenuifoliose A, tenuifoliose B, tenuifoliose J, polygalaxanthoneâ…¦and polygalaxanthoneâ…¢.Nerve cell regeneration is the key to treatment of depression. The isolated compounds were subjected to bioactive screening assay by in vitro cell model using H2O2-induced SH-SY5Y cells. The result showed that 3,6'-disinapoylsucrose and tenuifoliside A exhibited protective effect of nerve cells in low dose, while injury in high dose.In order to prepare enough sample to study the mechanism of pharmacology and pharmacokinetic of 3,6'-disinapoylsucrose and tenuifoliside A, a preparative HSCCC method was established. The isolation and purification procedure was performed using a two phase solvent system composed of ethylacetate-n-butanol-water (4:1:5, v/v/v) at a flow rate of 10.0 mL/min, when the rotation speed and the detection wavelength were set at 1200 r/min and 280 nm, respectively. From 400 mg of crude sample,3,6'-disinapoylsucrose (34.6 mg,93% in purity) and tenuifoliside A (65.2 mg,95% in purity) were obtained.Myricitrin posses a variety of pharmacological activities, but its structure is unstable. HSCCC was also employed to isolate and purify myricitrin. Two-phase solvent system composed of petroleum ether-Ethy acetate-methanol-water (3:4:4:3, v/v/v/v) was used at a flow rate of 3.5 mL/min, while the apparatus rotated at 850 r/min and the effluent was detected at 280 nm.251.5 mg of myricitrin was obtained from 500 mg of the crude extract of Myrica ruba tree bark with purity of over 98% analyzed by HPLC.In addition, advances in the research of chemical constituents, pharmacological activities and quality control of Polygala tenuifolia were reviewed. Furthermore, this paper also summarized the application of HSCCC in the reseach of Chinese traditional medicines.