BFGF Regulates Expression Of β-catenin In The Cardiac Fibroblasts Of Rats

Objective The Wnt signaling pathway has an important function in cell proliferation and migration of cardiac fibroblasts during the wound healing process. It also plays a very important role in cardiac physiological and pathophysiological processes, such as its transduction pathway, the healing after myocardial infarction, the proliferation, differentiation and orientation of cardiac cell, angiogenesis/ neovascularization, cardiac hypertrophy and heart failure, the deposition of the extracellular matrix and so on. This study is aimed at the bFGF influence the function of Wnt signaling pathway in cardiovascular hypoxia/reoxygenation condition.Methods To investigate the expression ofβ-catenin and explore the regulative effect and mechanism of basic fibroblast growth factor (bFGF) toβ-catenin protein in cardiac fibroblasts, we build cardiac fibroblast hypoxia/reoxygenation(Hy/R) model. Isolate the cardiac fibroblasts (CFs) from 1-3days SD rat ventricle. Then cardiac fibroblasts were cultured in 6 wells plate in normal condition and then in hypoxia/reoxygenation (Hy/R) condition for 24 hours. We use the forth generation cardiac fibroblast and divided into 6 groups (density 1×105/ml/well), including the normal control group (group 1), The hypoxia/reoxygenation (Hy/R) group(group 2), the 5μg/L bFGF+hypoxia/reoxygenation (Hy/R) group (group 3), the 10μg/L bFGF group (group 4), the 20μg/L bFGF group (group 5), the 40μg/L bFGF group (group 6). The MTT method was used to investigate the proliferation of cardiac fibroblasts, The expression ofβ-catenin protein and apoptosis cell were detected and measured by immunofluorescence method, laser confocal scanning microscope method and TUNEL method.Results MTT A490nm value of the bFGF groups was significantly increased vs Hy/R group (P<0.05, n=6). Under the laser confocal scanning microscope, the expression ofβ-catenin in bFGF group3,4,5 was increased significantly vs Hy/R group (P<0.05, n=6), The expression of P-catenin protein in cardiac fibroblast was significantly increased after hypoxia/reoxygenation(Hy/R), the expression ofβ-catenin in bFGF group was increased vs Hy/R group, but the cell apoptosis was decreased in bFGF group2,3,4,5 vs Hy/R group (P< 0.05, n=6).Conclusion The results indicate that bFGF can depresses cell apoptosis, participating in the regulation of the expression of P-catenin in the cardiac fibroblast, facilitate cell proliferation after Hy/R.