Studies On The Chemical Constituents Of Delphinium Grandifloru Linn

Delphinium grandiflorum Linn, a herbal drug, is a flowering plant belonging to Delphinium genus. Biological activity studies performed on this species have shown it has analgesia, anti-inflammatory, antiarrhythmic, local anesthetic activities and so on. Delphinium grandiflorum Linn is a perennial herb, with the height of 35-65 centimeters. The date of flowering period is August to September and fruiting period is September to October. The best harvest seasons are autumn, winter and early spring. It is distributed in Xinjiang, Shanxi, Hebei and Inner Mongolia and mainly grown on hillside, hollow, roadside, dene and tussock.In order to explore much more chemical constituents of Delphinium grandiflorum Linn, and to expoit new resources of Chinese Traditional Medicine.The chemical constituents of Petroleum ether, CHCl3, EtOAc and n-butanol fractions of EtOH extraction were isolated by means of silica gel column chromatography (CC), preparative thin layer column (PTLC), Sephadex LH-20, polyamide and recrystallization etc respectively, and 40 compounds were obtained; the structures of 34 compounds were elucidated on the basis of spectroscopic methods:IR,EI-MS,HR-ESI-MS,HR-EI-MS,1D NMR(1H NMR,13C NMR,DEPT,NOE,TOCSY), 2D NMR (1H-'H COSY,HSQC,HMBC,NOESY), along with comparison with authentic samples and the data from literature. They were identified as diterpenoid alkaloids, flavonoids, and steroids, coumarin etc, three of which were new compounds.In the end of the thesis, one review was made on the systemical summarization for the diterpenoid alkaloid constituents of the family of Ranunculaceae reported in the past ten years.The results of the investigation were expected to enrich the database of natural products, to afford the scientific basis of pharmacology, chemotaxonomy and screening of new medicines, and to help the utilization of the Chinese natural resources as well as the research of Chinese Traditional Medicine modernization.