Effects Of Restraint Stress On Glucose Metabolism And The Ameliorative Effects Of Carnosine In Mice

Object:Study on the effects of restraint stress on glucose metabolism and the ameliorative effects of carnosine in mice.Method:(1) Kun Ming (KM) mice loaded with restraint stress were induced by fixed in the restraint cage. Animals were randomly divided into normal group, stress group. After oral administration 2 g/kg glucose into the 20h stress mice, the elimination rate of plasma glucose, glycogen and plasma corticosterone were determined. (2) The KM mice were randomly divided into normal control group, stress control group,150 and 300mg/kg carnosine group, which were administered samples once a day successively for 7 days. After oral administration 2 g/kg glucose into the 20h stress mice, the elimination rate of plasma glucose, glycogen, plasma corticosterone and the mRNA expression of GY-2,G6PC,GK,IR,GR,GLUT-2 were determined.Result:(1) As compared with the control groups, stress groups decreased the basic glucose metabolism of plasma and glycogen. And it elevated the plasma corticosterone. (2) As compared with the stress groups, carnosine accelerated the basic glucose metabolism of plasma, elevated the glycogen synthesis in stress mice, and it also reduced the plasma corticosterone. In addition, carnosine up-regulated the mRNA expression of GY-2,GK,IR,GR,GLUT-2, but it reduced the mRNA expression of G6PC.Conclusion:Restraint stress decreased the basic glucose metabolism of plasma and glycogen. And it elevated the plasma corticosterone. Carnosine accelerated the basic glucose metabolism of plasma, elevated the glycogen synthesis in stress mice. The mechanism might be related to the degression of glucocorticoids and the up-regulation the mRNA expression of IR,GR. It also might be related to regulation of the expression of the key enzyme gene of glucose metablism and the improvement of the glucose transport.