Study On Chemical Constituents From Gorgonian Paraplexaura Sp.

Gorgonian is a rich source of bioactive substances with intriguing and unique structural feature as well as potential pharmaceutical applications. So further research in them will be of great significance in chemical theory and application in terms of enriching the knowledge of Natural Products Chemistry, discovering new medicines against disease and boosting the development of basic theory and synthesis in Organic Chemistry, it also established a foundation for futher development of other marine organisms with abundant resoucres in China. The coral Paraplexaura sp. belongs to class Anthozoa,order Gorgonacea and family Plexauridae, to the best of our knowledge, this is the first report of the chemical constituents and biological activities from the geneus Paraplexaura sp., this paper systematically investigated on the chemical constituents of the gorgonian Paraplexaura sp..The EtOAc and n-BuOH extracts of the gorgonian Paraplexaura sp. was chromatographed on TLC, silical gel, Sephadex LH-20 and semi-preparative HPLC, respectively, then sixteen compounds were isolated and purified from the gorgonian Paraplexaura sp., fourteen compounds were identified on the basis of spectrual methods(EI MS, ESI MS,1D-NMR,2D-NMR, ect), there were eleven compounds from EtOAc extract, four compounds from n-BuOH extract. All of these compounds were isolated from this specimen for the first time,and one was new 1-O-β-glucopyranosyl-(2S,3R,4E,8Z)-2-[(2-hydroxyicosanoyl)amido]-9-methyl-4,8--heptacosanediene-1,3-diol (11b), other compouds were identified as 2,4-Dichloro-benzoic acid (1), batyl alcohol (2), cholest-4-ene-3,6-diol,(3β,6β)-(3), N-2-(1,3-dihy--droxyoctadecan-4,8-dieny)-hexadecamide (4), thymine (5), 1,3-Dimethyl-2,4-pyri-midinedione (6), uridine (7), caffeine (8), aminophylline (9), thymidine (10), N-[1-[β-D-glucopyranosyloxy)-methyl]-2-hydroxy-8-methyl-3,7-heptadeca-dienyl]-2-hydroxy-(11a), tetradecanoic acid (12a), pentadecanoic acid (12b), octanoic acid (12c), cyclo-(Val-Pro) (13),4-(2-aminoethyl)-N,N-diethyl-aniline(14).