| Objective:To investigate effect of target Hes-1 siRNA on the differentiation of Th17 cells. Methods:Cell suspensions were prepared from mouse lymph nodes, total RNA was extracted by Trizol. The expression of Hes-1 mRNA and Deltex-1 mRNA of peripheral T lymphocytes was checked by RT-PCR method. The expression of protein Hes-1 was checked by Western Blot. The expression of IL-17 of peripheral T lymphocytes was checked by Elisa method. The expression level of intracellular IL-17 and IFN-y in peripheral CD4+T lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique. Results: Jagged-1 can promote the expression of Hes-1 mRNA, and the role of the third day was the most obviously. Its role was reduced on the fifth day. The expression of Hes-1 mRNA in DAPT group was significantly lower than the control group. The expression of Deltex-1 mRNA in Jagged-1 group was significantly increased, peaked at the second day. On the first day, the expression of Deltex-1 mRNA in DAPT group was lower than the control group, The second day began to increase. Hes-1 protein was not affected by Jagged-1 and Hes-1 siRNA effect 12 h. The expression of Hes-1 protein began to increase in Jagged-1 group after 24 h, and it reached peak at 36 h. The expression of Hes-1 protein was reduced after 72 h. The expression of Hes-1 protein decreased to a minimum at 36 h and continued to 48h in siRNA groups.100 nmol/L Hes-1 siRNA could significantly decreased the expression of Hes-1 protein, and had nothing different with 200 nmol/L,250 nmol/L Hes-1 siRNA groups. Flow results showed, Hes-1 siRNA promoted the expression of IL-17 in peripheral CD4+T lymphocytes. Jagged-1 decreased the level of IL-17 in peripheral CD4+T lymphocytes. Conclusions:Jagged-1 activated the Notch pathway, and decreased the expression of IL-17 secreted by peripheral CD4+T lymphocytes. Hes-1 siRNA interfered Notch pathway and promoted peripheral CD4+T lymphocytes to Th17 cells.
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