Expression Of GRP78 MRNA In Visceral Obese Tissue Of Rats Fed With High Fat Diet

ObjectiveTo establish insulin resistance rats model and observe the expression of GRP78 mRNA in visceral obese of rats fed with high fat diet in order to explore its effects on insulin resistance.MethodsThirty Wistar rats were randomly divided into two groups:normal chow group (NC), was fed with free normal chow for 10 weeks; high fat group (HF) was fed with high fat diet for 10 weeks. The expression of GRP78 mRNA was detected by real time PCR. We also detected total cholesterol, triglyceride, the rate of visceral fat (epididymal fat) vs. weight, fasting insulin.Results(1) Glucose infusion rate (GIR) in HF group was obviously lower than that in NC group(0.904±0.155vs.4.972±0.678mg-kg-1-min-1,P<0.01).Rats in HF group had an elevation of fasting insulin compared with NC group(15.871±1.966 vs.13.939±2.971ng/dl,P<0.05).(2) The expression of GRP78 mRNA was significantly higher in HF group than that in NC group. (6.155±6.799vs.1.212±0.644, P＜0.01).(3)Rats in HF group had an obviously elevation of triglyceride (0.292±0.108vs. 0.143±0.072mmol/l,P<0.01) compared with NC group. The rate of visceral fat (epididymal fat) vs. weight in HF group was higher than that in NC group (0.021±0.0 04vs.0.014±0.004g/g, P＜0.01)ConclusionAfter 10 weeks high fat diet, we establish insulin resistant animal models. High fat diet can induce the expression of GRP78 mRNA in visceral obese tissue.