Sources Of Non-liver Abscess Of Klebsiella Pneumoniae Serotypes Not In K1,K2 Main Type Objective

ObjectiveKlebsiella pneumoniae is the most common clinical Gram-negative bacteria, is causing hospital-acquired urinary tract infections, hospital acquired pneumonia and abdominal infections important pathogens. In recent years, the study found Asian community-acquired Klebsiella pne umoniae infection in another form of invasive infection, primary liver abscess with bacteremia, and Klebsiella pneumoniae serotype K1, K2-type main disease.Klebsiella pneumoniae capsular thick, rich in sugars, so that it can fight and phagocytosis by neutrophils and disease, but also has anti-serum bactericidal (ie the complement system) capability, it is Klebsiella pneumoniae capsule virulence factors of pathogenic bacteria. According to capsular polysaccharide antigens of different structure and can be divided into more than 80 Klebsiella pneumoniae capsular serotypes. Where K1, K2 Klebsiella pneumoniae anti-type neutrophils and phagocytosis and intracellular killing effect against stronger than non-K1, K2 type, therefore, K1, K2-based prevalence of high toxic. Currently on the susceptibility of Klebsiella pneumoniae, drug resistance and phenotypic detection of ESBLs reported more areas, while serotype detection, distribution and research rare. This study investigated Klebsiella pneumoniae serotype distribution and the distribution of clinical disease of Shengyang Liaoning Province.MethodsThe first affiliated hospital of China medical university selected microorganism room in 2008 to 2009 October identification and preservation of 149 strains Klebsiella pneumoniae isolates, including 42 from sputum,37 from blood,24 sources in urine,13 from pus,11 from discharge, nine from drainage fluid, seven from bile, six from other (including pleural effusion, a catheter, ascites, cerebrospinal fluid the two). After many generations, and the ability to extract DNA, DNA polymerase chain reaction (PCR) to detect Klebsiella pneumoniae serotypesKlorK2. And clinical data analysis combined with clinical disease in the liver abscess and non-liver abscess in the distribution of disease.ResultClinical data found that only 11 was from the liver abscess of the 149 specimens,accounted for 7.38%. But from other diseases, including respiratory diseases, circulatory diseases, digestive diseases, blood diseases, urinary system diseases, nervous system diseases. (For example, the splenic space-occupying lesions co-infected, gangrene of secretions, soft tissue infections, Necrotizing Fasciitis, gallbladder stones with cholecystitis, acute peritonitis, cerebral hemorrhage in patients with urinary tract infection or lung infection, intracranial infections and cancer patients with various site infection) That non-liver abscess 138, accounted for 92.62%.After detection of 149 strains Klebsiella pneumoniae specimens, K1, K2 of the positive rate was 3.36% include3 lines to serotypesKl,2 strains to serotypes K2.The serotypesKl are derived from the gallbladder stones, chronic cholecystitis bile culture; cirrhosis, spontaneous bacterial peritonitis in patients with blood culture and aspiration pneumonia patients of phlegm. The serotypes K2 are derived from diabetes patients with perirectal abscess of the nongzhi culture and spleen cavernous hemangiomas patients drainage broth. The other specimen is neither serotype K1 type, nor serotype K2 type. The 138 non-liver abscess from Klebsiella pneumoniae was detected in 133 non-K1, K2, their share as high as 96.38%ConclusionThese findings suggest that non-liver abscess in this region the source of Klebsiella pneumoniae serotypes not in K1, K2-based.