Efficiency Of Ketamine On Nuclear Factor Kappa B Activities Is Influenced After Ozone Preconditioning In Sepsis-induced Liver Injury In Rats

ObjectiveTo investigate the interaction between ozone oxidative preconditioning and ketamine on cytoplasmic NF-κB levels in endotoxic shock model(rats treated with lipopolysaccharide (LPS)).MethodsForty healthy Wistar rats were randomly divided into 5 groups (n=8 each):LPS treated and LPS-pretreated rats were administrated respectively with equivalent amount of 0.9% saline and 5mg/kg ketamine followed by infusion with 20mg/kg LPS.An equivalent amount of 0.9% saline was injected in the control rats.Following mixture gas of 5%O3 and 95% O2 15μg/kg injection for 5 days,20mg/kg LPS was infused after 24 hours in the group O3/O2+LPS+KT and O3/O2+LPS group,and then 5mg/kg ketamine and equivalent amount of 0.9% saline was injected respectively.In addition to histological examination of the liver, the activity of NF-κB was determined by SABC immunohistochemistry and was measured in each group respectively.ResultsHistological damage occurred in LPS group. It was characterized by hepatic arrangement turbulence, hepatic lobules distortion, congestion in liver sinusoids, hepatocyte swelling or necrosis, and granulocytes infiltration. These changes were not markedly obvious in 03/02+LPS+ketamine group.Normal control group has quite a low activity of NF-κB, but the activity of NF-κB was markedly increased in the LPS group(P<0.05). The NF-κB level was significantly decreased in the O3/O2+ LPS+ketamine group (P<0.05)when compared with the LPS+ketamine group,almost equal to control group.ConclusionThe preconditioning effect of ozone seems to support the biological effectiveness of ketamine by altering the nuclear factor kappa B activity,which may play an important role in sepsis-induced liver injury in rats.