| Infected nonunions are difficult cases for orthopedic surgeons. Recent studies focusing on the use of antibiotics and growth factor delivery systems to treat infected nonunions have become a major focus of orthopedic research. We studied the biodegradable materials Osteoset(?) for local delivery of vancomycin and recombinant human bone morphogenetic protein-2 (rhBMP-2) in vitro. Results of high performance liquid chromatography (HPLC) and bacterial inhibition tests indicated that the capsules release high concentrations of active vancomycin for up to 144 hours. Enzyme linked immunosorbent assay (ELISA) and alkaline phosphatase (ALP) tests indicated that active rhBMP-2 was released for up to 30 days. Furthermore, MTT tests showed that the capsules had no inhibition effect on the proliferation of mesenchymal stem cells (MSCs), indicating the biologically safty of the capsules. These results indicated that these capsules had very good prospect for clinical use.
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