| Many epidemiologic studies had reported extremely low frequency magnetic fields (ELF MF) may increase the prevalence rate of cancer.ELF MF was labeled into the category "2B" meaning possibly carcinogenic to humans by the International Agency for Research on Cancer (IARC) at 2001. However, this is still a controversial issue because of lacking enough and direct evidences from in vivo and in vitro studies.Occurrence of cancer cells is a multi-stage process involving invasion into surrounding tissue, intravasation, transit in the blood or lymph, extravasation, and growth at a new site. Many of these steps require cell motility and proliferation which involve the reorganization of actin into stress fibres, long filaments that traverse the cell, and focal adhesion complexes linking the cell to the extracellular matrix. It had been found that in many kind of tumors actin filaments appeared to be a lot of modification or expression, and the activity or expression of some important signal molecules were regulated.The aim of the present study was to investigate the effects of ELF MF at different densities and exposed time on F-actin cytoskeleton, Surface morphology, migration, proliferation and possible mechanism in Chinese hamster lung (CHL) cells. Scanning electron microscopy (SEM) and confocal laser scanning microscopy were used to investigate changes of cell surface morphology and F-actin cytoskeleton induced by ELF MF. Wound healing assay and MTT test were using to detect effects of ELF MF on cell migration and proliferation. The PI3K and phosphorylation-Akt (p-Akt) proteins were investigated by Western blot.The results suggest that ELF MF could induce reorganization of F-actin cytoskeleton and morphological changes, increased cell migration. The magnetic fields however didn't affect cell proliferation and expression of PI3K and p-Akt in CHL cells under the present experimental conditions.
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