| The surface plasmons resonance (SPR) sensor owns many important characteristics, which are its versatility and capability in real time monitoring the association or dissociation of biomolecules on the surface of the sensor without the need for fluorescence or labeling of the biomolecules. These characteristics made the SPR technique an easy, convenient and reliable one for determining the concentration and molecular weight, monitoring change in structure, measuring kinetic constant and binding specificity of individual biomolecules. Currently, SPR technology has been made a large number of results in biomedical research, such as analysis of protein interaction with DNA, drug identification and screening, study on interaction of antigen-antibody, determination of the virus, etc. Lyme disease was described as a new entity in the United States in the late 1970s. It is a zoonotic disease known as the "second AIDS", which can damage the nervous system, skin, cardiovascular system and joints and other organs of humans or animals. The above characteristics and the rapid development of SPR technology can make it develop into an effective, rapid and inexpensive technical means to carry out diagnosis of Lyme disease.Several couplers in the SPR sensors have been employed. These include fiber optic SPR sensor, grating-based SPR sensor and prism-based SPR sensor. Based on detection mode, the SPR sensors include angle modulation, polarization state modulation, intensity modulation, phase modulation and wavelength modulation. These setups were fully discussed in Chapter one. At last, some applications and researches of SPR technology on biomedical from different aspects were listed.In chapter two, a novel optical chemical sensor was developed. Traditionally, wavelength modulation SPR sensing method was to use tungsten lamp as light source which needed an expensive and complicated apparatus. The device presented here is a relatively simple and inexpensive. It was designed using white LED as light source, and lightened by a 5V battery. The setup consisted of white LED, light transmit system, sensing element, flow cell, detection system and data dealing system. The SPR sensor was designed on the basis of fixing the angle of incident light and measuring the change of resonant wavelength. The light emitted from the white LED is polarized to give TM polarized light, and two lenses are employed to make the light parallel. The parallel polychromatic light beam passes through an optical prism with a thin gold film on the bottom and excites surface plasmon at the interface between the gold film and solution. The output light from the prism is guided into a charge coupled device (CCD) detector by fiber. The data acquired by CCD was processed by data processing system. To further reduce the experiment cost, some improvements on sensing devices and flow cell were carried out. We depositted the gold film on the coverslip, which will be pasted on the bottom of prism with coniferale oil, insteading of gold film in the prism directly.The study on Lyme disease spirochetes were presented in chapter three. First, three kinds of anti-serum of Borrelia burgdorferi were get by rabbit immune trial, including PD91 antiserum, FP1 antibody and B31 antibody. The three genotypes of Lyme disease spirochetes were detected by biological self-assembly analyzer based on surface plasmon resonance. Through these experiments, we can got kinetic curve between of different antigen-antibody reaction, and their kinetic constants will be worked out. That the test used SPR technology in detecting Borrelia burgdorferi is flexible and easy to operate, saving time and drugs, high sensitivity, with good prospects. |
