| Cervical cancer is one of the most common cancers affecting women, which's disease incidence is only lower than breast cancer. In Chinese, cervical cancer's disease incidence is at the first place in gynecological malignancies, and which trends to younger. Now disease incidence and mortality rate is at a high level. We have known that the occurrence of cervical cancer connects firmly with HPV, but now we still research its pathogenesis hotly in molecule level in gynecological field.As early as 1972, Kerr and others had proposed the concept of apoptosis, but until the recent 10 years, we made an rapid progress in it by deep research. At present, the detail molecular mechanism abort apoptosis have been reported in many literatures。Now we can't define all problem about tumorous by apoptosis, but more and more literatures suggest that apoptosis is regulated by gene. Apoptosis direct active cytocidal in vivo, which is necessary with kinetic equilibrium of cell proliferation to keep up with growth and development,homeostatic equilibrium and immune omodulation; It is associated tightly with tumorigenesi. Tumor is a kind of disease that is connated with abnormal generation and disdifferentiation, but also with abnormal apoptosis. The loss of control of apoptosis isassociated tightly with canceration. There is much research about apoptosis in the process of cervical cancer. We detected apoptosis cell in cervical cancer, by which we hope to approach apoptosis in cervical cancer and connect with clinical stage and pathology,and to offer theory for clinical diagnosis, treatment and the judgement of prognosis.Thirty women'cervix tissue are taken as the research objects who have received health examinations in the maternity department in the second clinical hospital attached to Jilin University, among which 20 women who got cervical cancer belong to the experiment group, and the other 10 women who didn't cervical cancer belong to the control group. According to the diagnostic standard of CIN, the 20 women who got cervical cancer was divided into two groups, among which 12 women who got CIN I, the other 8 women who got CIN II/III. All the 20 women's cervix tissue of cervical cancer were confirmed by pathology,among which 16 women belong to cervix prickle cell carcinoma, and the other 4 women belong to adenoca. All the 20 women who got cervical cancer were the first cases, and didn't accept any therapia such as chemotherapy, chemotherapy or chemotherapy. According to the diagnostic standard of cervical cancer amended in 2003 by FIGO, all the 20 women who are divided into three groups, among which 3 women belong to IA,and 5 women belong to IB, and 12 women belong to IIB. In pathology, Broder classification was adopted (well-differentiated G1, moderately differentiated G2, poorly differentiated G3). Cervical specimen were made into monoplast cell suspension with requisition of flow cytometry, and adopted for AV/PI double-staining method. We detected all kind of cell subpopulation in the monoplast cell suspension by flow cytometry and calculated apoptosis's percentage in the monoplast cell suspension. Using the software of Spss13.0 in Statistics, average +standard deviation(x+s) is adopted in the measurement data, and data of each group is examined by independent sample t-test. The analysis of dependability adopts Spearman interclass correlation. If p<0.05, we thought there was different in statistics.After detected by flow cytometry , all the monoplast cell suspension was divided into three cell subpopulation, including living cell, apoptosis cell and dead cell. The living cell membrane was integrated and there was no externalization in PS, and it displayed for AV-/PI-. The apoptosis cell membrane was also integrated ,but there was externalization in PS, and it displayed for AV+/PI—. The dead cell membrane was disintegrated and there was externalization in PS, and it displayed for AV+/PI+. In the control group, there was a great quantity of living cell and the percentage of living cell was (91.44+1.19)% which was higher than CIN I group, and there was difference in statistics; While there was little dead cell and the percentage of dead cell was (2.98+1.30)%; The percentage of apoptosis cell was (5.30+0.70)%. The result of control group was accord with the cytokinetics characteristic of normal tissue. In the CIN I group, The percentage of apoptosis cell was (7.60+0.78)%, and there was difference in statistics compare to normal control group(p<0.05). In the CINⅡ/Ⅲgroup, The percentage of apoptosis cell was (17.96±3.11)%. From CIN I to CIN II/III, the percentage of apoptosis cell increased, and there were apparent difference in statistics between them (P<0.05);While from CIN II/III to the stage of invasive carcinoma of cervix, it decreased and kept at low level, it was (8.60±2.49)% which was obviously lower than the CINII/III group, and there were difference in statistics between them(P<0.05); In this experiment, we found yet that the percentage of apoptosis cell in tumor-adjacent tissues was higher than that in cancer tissue, while the percentage of dead cel in tumor-adjacent tissues was lower than that in cancer tissue, and there were apparent difference in statistics between them (P<0.05). The percentage of apoptosis cell in clinical II stage of cervical cancer was lower than that in clinical I stage of cervical cancer, but there were no difference in statistics between them (P>0.05). The percentage of apoptosis cell in squamous carcinoma of the cervix group was higher than that in adenocarcinoma of the uterine cervix group, and and there were difference in statistics between them (P<0.05). In the classify of histology, the percentage of apoptosis cell in the well-differentiated group was higher than that in poorly differentiated group, and there were difference in statistics between them (P<0.05). The percentage of apoptosis cell in the cavitas pelvis lymph node metastasis group was lower than that in no cavitas pelvis lymph node metastasis group, and there were difference in statistics between them (P<0.05). Compared to other directions, such as the size of gross tumor volume, the shade of cervix uteri infiltration, whether side uterus infiltration or not, whether vessel to be involved or not , there were no difference in statistics(p>0.05).To sum up with all the result, we made conclusions that from CIN I to CIN II, and from CIN II to CIN III, the degree of cell atypical hyperplasia aggravated gradually, and the percentage of apoptosis cell increased gradually too. There was a positive correlation between the degree of CIN damage and the percentage of apoptosis cell. These was maybe consistent with the occurrence of multi-step theory of cervical cancer, which suggest that with the strengthen of cell proliferation, the organism keep a balance by guiding apoptosis. These means that with the strengthen of cervix uteri epithelial cell proliferation, apoptosis increase too, and the organism keep a dynamic balance, not leading to being a cancer. At the stage of invasive carcinoma of cervix, the percentage of apoptosis cell degrade obviously, and keep at a low level, which suggest that the organism's ability be weaken by inducing apoptosis and wouldn't be able to keep a balance with cell hyper-proliferation. The coodination between apoptosis and cell proliferation have been confused, and lead to being a cancer finally. According to the result, we guess that in the development of uterine cervix carcinogenesis, apoptosis maybe work in some point in earlier period only, which provide some theoretical proof that we apply drug treatment promoting apoptosis in the earlier period of cervical cancer. There was a correlation between apoptosis and pathologic types of cervical cancer, degree of tissue differentiation, whether or not diversion of nodus lymphaticus. Up to now yet, it is not full and there is no final conclusion to study and research the relation between apoptosis and clinical prognostic. It would be an extremely useful supply to lead the concept of apoptosis in the standard of evaluating prognosis and treatment effectiveness evaluation. By studying and researching the apoptosis in cervical cancer and approaching the effect of apoptosis in the earlier period of cervical cancer, we found if we apply drug treatment, it would interrupt the formation of cervical cancer. If above-mentionde theory should be applied, it would develop a new way in the aspect of cervical cancer's diagnosis, therapeutics, prognosis and so on.
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