| Objective:This study firstly successfully established the simple obesity model in male pubertal rats fed up from 3-week-old ones. To investigate the effects of pubertal simple obesity on the reproductive system in male animals, we observed the reproductive organs developments, pathological changes and endocritic adjusting to investigate the effect of obesity on the reproduction of male animals, we also observed the situation of copulation, ingravidation and fetuses after sexual maturity and reducing weight of male rats. Through these studies we hope to understand the main mechanism of pubertal obesity on reproductive system of male animals or do some preparation of the deeper investigation, and to indicate the severity of the effect or injury and wether it is reversible.Method:1. Fifty 3-week-old male rats were randomly divided into a control group ( n = 20) and an fat-model group ( n = 30) , and fed respectively on a normal diet and a high-fat diet.2. Monitoring the body weight, Lee's index and surrounding fat of kidney and testis can determine wether the simple obesity model is successfully established.3. Weight the testis and parorchis, and calculate their organs coefficients.4. Observe the changes of parorchis, vesicula seminalis, prostate and testis in light microscope. 5. Mensurate T and E2 in blood use ELISA.6. Examine the amount of apermatozoa and their activity ratio.7. Examine the reproduction of the fat male rats in the inseminatio rate, conception rate of female rats, numbers and gender of foetus.8. Observe the inseminatio rate and the change of reproductive organs after reducing weight.Result:1. Establishing the simple obesity model in male rats: By the fourth weekend, the body weight, Lee's index and surrounding fat of kidney and testis were obviously higher than the control group.2. Impact of obesity on the reproductive organs developments of male rats: The weights of all the checked organs were higher than control group, especially obviously in parorchis, prostate and vesicula seminalis. Light microscope examination showed that compared with the controls, real cells of parorchis, vesicula seminalis, prostate and testis reduced, instead of generous interstitial substance and vacuolus especially in testis cells (the spermatogenic cell layers decreased and arranged in disorder).3. Effect of obesity on the hormone in blood: In some degree, T and E2 were higher than controls.4. Effect of obesity on quality of apermatozoa: Compared with the controls, the amount, abnormity rate and activity ratio declined.5. Impact of obesity on reproduction of male rats: Only the femal conception rate of fat-model group was obviously lower than the control group. Other indexes showed that the reproduction of fat male rats injuried in some degree.6. Effect on inseminatio rate after reducing weight: After successfully reducing weight, inseminatio rate increased. But the weights of testis and parorchis were still dramatically higher than controls the organs coefficients were obviously lower compared with control group. Discussion:Simple obesity indicates a clinic disease which dues to fat proliferating excessively in all of cells. It has no relationship with some genetic diseases, metabolic diseases and endocrinous diseases.In order to study the effect of obesity on reproductive system in the male, first step is to establish the fat-model animal. By the end of the fourth weekend of our feeding rats, we have found that the average weight of fat-model group is obviously higher than control's. By the end of the whole feeding, it increased 86.56% compared with the control group. Aside body weight, Lee's index and surrounding fat of kidney and testis can also help us determine the simple fat models exactly.It is important that reproductive system of male develops in the puberty. Sexual hormones in blood may response the developments of genitical glands in some degree. Obesity can change the consistency of T and E2. In our study, T and E2 increased, but not obviously, because of the limited sample size.Light microscope examination showed that compared with the controls, real cells of parorchis, vesicula seminalis, prostate and testis reduced, instead of generous interstitial substance and vacuolus especially in testis cells (the sperm- atogenic cell layers decreased and arranged in disorder). These morph- ological changes may due to the obesity itself and increasing of E2.When investigating the effect of obesity in reproduction of male rats, we found the reproduction was affected due to the dramatically declined conception rate of female copulated with the fat-model group.As a hormone product of OB gene, Leptin can adjust the amount of intake through HT and fat metabolism through autocrine. Leptin in obesity boy is significantly higher than it in normal boy, but T is lower. Therefore, puberty and sexual development delay in obesity boy. If he does some early exercises to reduce weight, it can be mitigated by increasing genetic expression of Leptin receptor to raise the sensitivity of Leptin. Conclusion:(1) Our studis successfully established the simple obesity model in male rats, which ingested the high-fat diet freely.(2) The weights of parorchis, prostate and vesicula seminalis of the model group were obviously higher. Compared with the controls, real cells of parorchis, vesicula seminalis, prostate and testis reduced significantly, and expresses of interstitial substance attenuated, instead of numbers of vacuolus. Organs coefficients of testical and parorchis depressed dramatically.(3) The amount, abnormity rate, activity ratio and inseminatio rate of obesity rats declined a little the femal conception rate of fat-model group was significantly lower than the control group.After successfully reducing weight, inseminatio rate had some improvements, but the organs coefficients especially testical and parorchis had not changed obviously.Conclusively, reproductive organs of male pubertal rats develop slowly because of simple obesity. Their real functioning cells usually reduce and thus the reproduction is decreased when sexual maturation. It is promisingly raised after weight reduction, but the developmental condiction of reproductive organs doesn't revive obviously. Now we haven't find out strong evidences for the impact of obesity male rats on foetus.
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