Effect Of Heptanol Preconditioning On The Expression Of Connexin 43 Gene In Myocardial Cellular Membrane Of A Myocardial Ischemia Reperfusion Model Rabbit

Objective:Myocardial ischemia reperfusion model rabbits were prepared.The present study was aimed to study the effect of heptanol preconditioning on the level of myocardial connextin 43 mRNA.After heptanol preconditioning thereby to investigate the possible mechanism of heptanol preconditioning in cardialprotection.Methods:A rabbit models of myocardial ischemia reperfusion injury was established.50 healthy rabbits were randomly divided into 5 groups:sham operation group(sham group,n=10),ischemia reperfusion injury group(I/R group,n=10),ischemic preconditioning group(IP group,n=10),heptanol preconditioning group(HT group,n=10),and heptanol preconditioning and 5-hydroxydecanoate treatment group(HT+5-HD group,n=10).Myocardial ischemia was induced for 30min followed by reperusion for Omin(Romin),30min(R30min).Celluar morph was analyzed by light microscope,CK-MB and cTnI were assayed by automatic biochemistry analyzer. The infarct area was detected by TTC assay.Thelevel of Cx43 mRNA in myocardium was analyzed by RT-PCR. Results:1.Heptanol group and IP group could significantly decrease infarct area and myocardial creatase level as compared to IR group,but with no statistical differences between them.2,It was observed that in Romin the levels of Cx43 mRNA in the ischemic area of IR group,IP group and Heptanol group were significantly lower as compared to sham values,but with a more significant decreasing trend with the reperfusion in IR group(P<0.05)and a increasing trend in IP group and Heptanol group(P>0.05);There was no significant difference between IP group and Heptanol group in the Cx43 mRNA level of ischemic area(P>0.05).3,It was observed that in R0min the levels of Cx43 mRNA in the ischemic area of Heptanol group, heptanol preconditioning and 5-hydroxydecanoate treatment group were significantly lower(P<0.05).Conclusion 1.Ischemic preconditioning and Heptanol preconditioning could significantly reduced the ischemia reperfusion injury.2.IR could decrease Cx43 mRNA level of ischemic area.3.Ischemic preconditioning and Heptanol preconditioning could increase the Cx43 mRNA level.4. 5-hydroxydecanoate could reverse the increase of Cx43 mRNA. mitoKATP may participate in the 5-hydroxydecanoate of ischemic preconditioning.The present study suggest that Cx43 may participate in the cardialprotection of ischemic preconditioning and Heptanol preconditioning.and the mechanism may invole the mitoKATP pathway.