G-CSF Induces Stem Cell Mobilization The Interaction Of VEGF And Ang/Tie-2

Mobilization of hematopoietic stem and progenitor cells from the bone marrow into the circulation by repetitive, daily stimulation with Granulocyte colony-stimulating factor (G-CSF) is widely used for clinical transplantation. Although adequate CD34+ cells were collected for a majority of patients by G-CSF, some patients and also healthy donors are still poor mobilizers. Therefore, It is necessary to explore the mechanism of stem cell mobilization.We included G-CSF mobiliezed healthy donors in our study. To investigate the mobilization effect of low-does granulocyte-colony stimulation factors(G-CSF) on allogeneic peripheral stem cell transplantation (PBSCT) through retrospective study. We investigate the change of VEGF,Tie-2, Ang-1,Ang-2 in the steady-state PB and mobilized PB and the correlation of the ratio and number of CD34+ cell of PBSC collections. We also investigate the change of Ang-1, Ang-2 in the steady-state BM and mobilized BM in order to explore the characterization and roles of Ang-1, Ang-2 in G-CSF induced mobilization.Result1. The ratio and number of CD34+ cell of PBSC collections of fifth day is more than the the fourth day (p<0.05). And the fourth day is more than the sixth day (p<0.05). Twice day (fourth day and fifth day) collection can obtain enough CD34+ cell for PBSCT.2. Lower Tie-2 concentrations in the plasma were observed after 5-day G-CSF administration compared to steady-state PB(p<0.05); higher VEGF concentrations in the plasma were observed after 5-day G-CSF administration compared to steady-state PB(p<0.05).3. The levels of Tie-2 has a negative correlation with the ratio and number of CD34+ cell of PBSC collections of fifth day(p<0.05); The levels of VEGF has a positive correlation with the ratio and number of CD34+ cell of PBSC collections of fourth day to sixth day.(p<0.05)4. After mobilization Ang-1 expression decreased compare to steady-state bone marrow by immunohistological staining of bone marrow in healthy donors.Conclusion1 The best day collect stem cell is the fifth day and it's better to collect stem cell on fourth day and fifth day rather than on fifth day and sixth day after mobilization.2 VEGF concentrations in the plasma was higher after mobilization in PB; Tie-2 concentrations in the plasma was lower after mobilization in PB. Therefore, VEGF and Tie-2 may regulate the mobilization of stem cell.3 Ang-1 expression decreased on fifth day in BM after mobilization, it suggested that Ang-1 promote mobilization of stem cell.