Research Of Qing Jie Huo Xue Tang On Experimental Autoimmune Retinal Vasculitis

ObjectiveThrough observing rabbit's retinal vascular disease which was induced by using bovine serum albumin, wo could recognized that some retinal vasculitis is an autoimmune disease and increase the pathogenesis of retinal vasculitis awareness. Studying the protective efforts to rabbit's retinal vasculitis, it may be valuable as therapeutic angents for retinal vascular disease especially retinal vasculitis.MethodInjected beneath the skin of Rabbit's feet of the mixture of which is equal-volume of 2% bovine serum albumin saline solution and Complete Freund's adjuvant, every time one foot was injected 0.1ml, co-injection four times and with an intervals of 7days. Intravitreal injection of bovine serum albumin saline solution(0.05ml) in the rabbit eyes after the 7 days of the last injection. After 24 hours, treat the blank control group with saline, treat Chinese herb medicine group with qing jie huo xue tang and positive control group received intraperitoneal injection of Dexamethasone, examine changes in clinical findings, fluorescein angiography, retinal vascular digestion, histopathologic finding, electron microscopy, histochemical changes, enzyme-linked immunosorbed assay and computerized image analysis when the treating was got for 7 and 14 days.Result1 Ophthalmoscopic studies of the retina after intravitreal injection of bovine serum albumin demonstrated that retinal hemorrhage, edema, venous segment liking thick and tortuous, local vessel becomes narrowing, optic disc swelling, ocular pigmentation changes, vitreousopacitees and so on. The showings of fundus fluorescein angiography were that hyperfluorescence of the optic disc, vascular occlusion, prominebt vascular nonperfusion throughout the retina, small foci of leakage from some retinal blood vessels and so on. Light and electron microscopy observed that the retinal pigment epithelium cells and retinal photoreceptor cell layer, followed by the ganglion cell layer and nerve fiber layer.2 The high dosage of the traditional herbal drugs was more effective to this model, and it can effectively, reduce bleeding, relieve inflammation and reduce the expression of retina ICAM-1. The state of illness will may be well better when the increasing treatment time., and efforts of low dosage weaker than the high dosage. Positive control group was significantly reduced edema, hemorrhange and inflammatory cells at the first 7days, but after the longer period, it aggravated inflammatory changes.Conclusion1 The model of the acute inflammation response which was induced by using bovine serum albumin had many common clinical signs and histophthologic changes with other ocular disease, especially the retinal vasculity. So it may be a good model.2 The pathogenesis of the experimental autoimmune vasculitis which was induced by using bovine serum albumin possibly the immunity compound deposition, and between the immunity commpoun-complement-neutral granular cell the ch ain-reaction is the immunity compound vasculitis pathogenesis center link; furthermore this vasculitis is antimmunological disease which maybe lied to one kind of cell leading.3 The experimental autoimmune vasculits which was induced by using bovine serum albumin is the acute inflammation response, and according to the pattern differentiation in traditional Chinese medicine, it's belonged to the pattern of heat toxin congestion and exuberance.4 Qing jie huo xue tang could partly protect the retinal and vasculars from bovine serum albumin, having no obvious side-effect. It will be of great value of clinical practices of these similar diseases.