The Expression And Their Correlation Of DPC4,P16 And Bcl-2 In Oral Leukoplakia And Scc

Objective: To detect the expression of DPC4,P16 and Bcl-2 in oral leukoplakia and squamous cell carcinoma(SCC), and to investigate the mutation and possible mechanism of DPC4,P16 and Bcl-2 in canceration of oral precancerous lesion and to discuss the correlation of DPC4,P16 and Bcl-2 further, which provided reference indexs for detecting canceration of oral precancerous lesion and early diagnosis,therapeutic options,prognosis of OSCC.Methods: Immunohistochemistry(polymer) was used to detect the expression of DPC4,P16 and Bcl-2 in 26 cases of oral leukoplakia and 30 cases of OSCC. Real-time fluorescent quantitative PCR was applied to detect level of DPC4 mRNA in 10 cases of oral leukoplakia and 10 cases of OSCC.Results:1. The expression of DPC4 protein is different respectively in 10 cases of normal tissue, 26 cases of leukoplakia and 30 cases of OSCC tissue, and the differences of them have statistical significance. (P﹤0.05)。the DPC4 mRNA levels is different in 10 cases of each group, and there were statistical significant differences among them.(P﹤0.05). The result of DPC4 dectected by the SABC was consistent with that detected by the real-time fluorescent quantitative PCR(Kappa=0.932>0.75).2. The expression of Bcl-2 protein is different respectively in 10 cases of normal tissue,26 cases of leukoplakia and 30 cases of OSCC tissue. There were significant differences among them. (P﹤0.05)3. The expression of P16 protein is different respectively in 10 cases of normal tissue,26 cases of leukoplakia and 30 cases of OSCC tissue. There were significant differences among them. (P﹤0.05)4. The expression of DPC4 protein was negative correlated with that of Bcl-2 protein (r = -0.827,P<0.05)and positive correlated with that of P16 protein(r = 0.569,P<0.05).Conclusion:1. The expression of DPC4 protein and the levels of DPC4 mRNA decreased gradually in normal tissue, leukoplakia and OSCC tissue indicated that DPC4 may play an important role in oral canceration. Less expression of P16 protein in OSCC tissue than in normal tissue and leukoplakia tissue implied that P16 may participated in oncogenesis and development of OSCCs. The expression of Bcl-2 protein increased by the augmented that degree of canceration alluded higher expression of Bcl-2 may be propitious to oral canceration.2. The expression of DPC4 protein was negative correlated with that of Bcl-2 protein, and positive correlated with that of P16 protein implied that either loss of DPC4 or low expression of p16 or overexpression of Bcl-2 or all may be involved in oncogenesis and development of OSCCs, which provided reference indexs for detecting canceration of oral precancerous lesion3. Both immunohistochemical assay and real-time fluorescent quantitative PCR can be used to detect the targeted genes. Compared to real-time fluorescent quantitative PCR, the immunohistochemical assay was easier to perform and more cost-effective, therefore immunohistochemistry can be used in the initial detection of the expression of protein of the targeted genes.