The Effect Of Ulinastatin On The Liver Function After Limb Ischemia-reperfusion In Rats

Objective To observe the protective effect of Ulinastatin on the liver function after limb ischemia-reperfusion in rats and the potential mechanism. Methods The developments of healthy SD rat hind limb ischemia-reperfusion model, 24 healthy male SD rats were randomly divided into three groups of eight. Sham-operated group, only line of anesthesia, heparinization, the right hind relaxation around the rubber band, non-blocking blood flow, I operate with the ischemia-reperfusion group, blood and liver tissue prepared to take inspection. Control group were anesthetized and heparin, rubber band ligation around the roots of the right hind, blocking blood flow 4 h after the lysis and restore blood flow 4 h after the blood and liver tissue prepared to take inspection. Experimental group, with the modeling, the vena dorsalis penis immediately before ischemia to be Ulinastatin 5×104 U / kg, total 0.5 ml, sham-operation group and the control group normal saline intravenously. The hepatic function and the blood serum of TNF-a,IL-10 were determined. The ultrastructures of mitochondria were observed by electron microscope. The constructions of hepatic were observed by light microscope.Results The liver function target determination, the control group, the experimental group compare sham-operation group ALT, AST, LDH to elevate, the experimental group compares control group ALT, AST, LDH to reduce, inflammation factor TNF-a, the IL-10 determination, the control group, the experimental group compare sham-operation group TNF-a to elevate, the experimental group compares control group TNF-a to reduce, the control group, the experimental group commissions array IL-10 to elevate, the experimental group compares control group IL-10 to elevate, has the significance difference (p <0.05). The guise of an array of mitochondrial structure: 0, control group 2-3 grade, the experimental group 1.Conclusions Ulinastatin has protective effects on the liver function after limb ischemia-reperfusion in rats. The theory is to decrease the blood serum of TNF-a and raise the blood serum of IL-10 ,so the inflammatory reaction is inhibited to protect the hepatic function and the ultrastructures of mitochondria.