Down-regulation Of ETS1 Mrna Expression In Peripheral Blood Mononuclear Cells From Patients With Systemic Lupus Erythematosus

Background Systemic lupus erythematosus (SLE) is an autoimmune disease which complexity of clinical manifestations. Its pathogenesis has not yet been fully clarified which generally believed that genetic, immunity, endocrine and environmental factors, genetic factors play an important role in the pathogenesis of SLE, which related to the development of SLE genetics faster. In the past two decades, many susceptibility genes of SLE had revealed through linkage analysis, candidate gene and the mouse model of lupus. Online Mendelian Inheritance in Man (OMIM) has collected 13 SLE susceptibility loci including 1q41-q42(SLEB1), 2q37.3(SLEB2),4p16-p15.2(SLEB3), 12q24(SLEB4), 13q32(SLEB5), 16q11.2(SLEB6), 20p12(SLEB7), 20q13.1(SLEB8), 1q32(SLEB9), 7q32(SLEB10), 2q32.2-q32.3(SLEB11), 8p23.1(SLEB12) and 6p23(SLEB13), and the last two susceptibility loci using genome-wide association analysis. In recent years, genome-wide association (GWA) study approach as the most effective way to find complex disease susceptibility genes, and a number of SLE susceptibility genes GWA studies had carry out.zhuangxuejun el at.had performed a genome-wide association study of SLE in a Chinese Han population and identified nine new susceptibility loci((ETS1, IKZF1, RASGRP3, SLC15A4, TNIP1, 7q11.23, 10q11.22, 11q23.3 and 16p11.2; 1.77×10?25≤Pcombined≤2.77×10?8) . SLE is a complex disease which is non-Mendelian inheritance patterns with strong ethnic differences and genetic heterogeneity. Objective To investigate the expression of the proto-oncogene E26 transformation-specific-1(ETS1) in the peripheral blood mononuclear cells(PBMC) of patients with systemic lupus erythematosus(SLE) and further investigate the roles and significance of ETS1 in the pathogenesis of SLE.Methods Generation of longer cDNA fragments from serial analysis of gene expression(SAGE) tages for gene indentification was applied to indentify the gene ETS1 according to the long SAGE tag. The real time-polymerase chain reaction (RT-PCR) technique was used to quantitatirely analyze mRNA expressing of ETS1 in PBMS from 61 SLE patients and 67 healthy subjects.Results Compared with controls, there are different between SLE patients and the health controls (p<0.05), the average level of mRNA expression in SLE group was lower than that in healthy controls.Conclusion These results indicate that significant difference between SLE patients and the health controls. The mRNA expression level of ETS1 in SLE group decrease markedly.