Protective Effect Of Hesperidin Against Hepatic Immune Injury In Mice And Its Mechanism

The essay used the immune hepatic injury models. It investigated the mechanism of its protective effect on immune hepatic injury from modulation of immunological function and the levels of cytokines production .Objective To study the protective effect of hesperidin against immune hepatic injury induced by concanavalin A in Mice and its mechanism..Methods All groups were injected with ConA once again with ConA (20mg/kg) from tail vein in order to induce immune hepatic injury except the mice in normal group. Animals were sacrificed at the 8th hour and blood was collected, the liver was harvested for liver homogenate. Index of liver were tested.The levels of ALT, AST, IL-1, IFN-γand TNF-αin serum, the contents of MDA, SOD, NO and GSH in liver homogenate were measured. the levels of TNF-αmRNA expression in liver were tested by reverse transcription polymerase chainreaztion(RT-PCR).Histopathlogical examination was observed. TNF-αand NF-κB p65 in hepatic tissues were determined by the method of immunohistochemistry,Results Compared with that of model group, the levels of ALT, AST, MDA, NO, IL-1, IFN-γand TNF-αwere significantly lowered by hesperidin whereas the levels of SOD and GSH were markedly increased by hesperidin and the hepatic pathological features were improved. Hesperidin could inhibit the expression of TNF-αand NF-κB p65 and TNF-αmRNA in liver tissue.Conclusion Hesperidin is effective in protecting hepatic lesions in mice induced by ConA. It is possibity related to removing free radicals, enhancing the antioxidant capacity of lipid peroxidation and reducing the levels of IL-1, TNF-αand IFN-γ.