Effect Of Procyanidins On The Retinal Photochemical Damage In Mouse

Objective:Observe the pathological features and cell apoptosis after retinal photochemical injury and to investigate the protection and rescue to retina of procyanidins.Methods:1. Fifty Kun Ming mice were randomly divided into five groups with 10 mice in each group: Group A(normal group),Group B(model group) and Group C (drug-treatment group). [Group C1(before operation),GroupC2(throughout operation),GroupC3(after operation)].2. Modeling methods:All of the mice except Group A were exposed to intermittent white light in a trunk with 12h dark adaptation and 12h light exposed at the light power of 16473.5±402.8 1x and the temperature between 28～32 degrees. Group C were given Procyanidins with dose of 400mg/kg. All of the animals were executed after 7 days. And the eyes were immediately took out and performed with Paraffin embedding, serial sections, HE and TUN EL staining.3. The morphological changes were observed and analyzed with optical microscope.4. The retinal cell apoptosis was showed by TUN EL method and counted.5. Statistical analysis:the mean and Standard deviation were calculated and analyzed with analysis of variance.Results:1. The outer nuclear layer retinal cells and the photoreceptor cells were distributed in order and had normal morphology in Group A.2. HE staining indicated that the model of retinal photochemical injury was successfully established. The photoreceptor cells were manifested as shortening of outer segments and reduction of cells in the cells.3. In the treatment group (Group C), the outer nuclear layer retinal cells and the photoreceptor cells were swelling but in normal morphology, and had obvious difference with Group B. 4. TUNEL assay shows large amount of TUNEL positive cells in ONL in Group B. and almost no positive cells in Group C, especially in C25. The statistics show that the thickness of ONL and AI had statistical significance between five groups (p<0.05).Conclusion:Procyanidins can protect and rescue the retinal and inhibit pathological cells apoptosis after retinal photochemical damage.