| The etiology of UC still remains incompletely understood, so far, more research continues to reveal the mechanism that UC is inflammation leaded by abnormal immune response which induced by intestine mucosal antigen with genetically susceptible people. with regards to this, In recent years, the treatment for UC is to adopt various methods mainly to focuse on the closure of mucosal inflammatory pathway, blocking the inflammatory process, so as to achieve the treatment goals. Studies have shown that, If we block the excessive immune response, or inhibit the certain cytokines, inflammatory mediators, may alleviate the condition. Meanwhile, the anti-inflammatory cytokine restrict the role of inflammation through the balance of pro-inflammatory cytokines in the progress of Immune response, the balance between was broken, can contribute to the occurrence of inflammation, therefore, if the activity of anti-inflammatory cytokines is increased,it can also alleviate the UC disease.ChangBiqing suppository was researched by our laborytory and is constituted by two kinds of materials with little side-effects but definite efficacy in anti-bacterial anti-inflammatory,4-amino-acid (4-ASA) and berberine based-hydrochloric (BBH).In this study, we treat thel UC model rats with ChangBiqing suppository, to investigate the effect of ChangBiqing suppository on ulcerative colitis in modle rats as well as on the expression of IL-1β,TNF-α,IL-10,SOD,COX-2,NF-κBp65 and TGF-β1 in colonic mucosa to reveal the possible mechanism for treatment.To investigate the effect of ChangBiqing suppository on ulcerative colitis in modle rats. SD UC model rats induced by dinitrochlorobenzene (DNCB)and acetic acid were randomly divided into five groups from A to E:UC model group(A), low dose intervention group(B), moderate dose intervention group(C), large dose intervention group(D), SASP group(E), another six rats as normal control group(F) besides. The fresh colons of the rats were got and examined after 14 days of treatment, meanwhile the DAI, HPS and CI were evaluated. Results:DAI and HPS of the rats in the model group were significantly higher than the control group(P<0.01). Compared with the UC group, the DAI,HPS in group B to E decreased markedly(P<0.01), but they still differ significantly from the control group(P<0.01); The HPS decreased in a dose-dependent manner from B to D and group B differed significantly from group C and D(P<0.01). In contrast, no significant differences in HPS were observed among the three groups C, D and E. Conclusions:ChangBiqing suppository demonstrated good results in prevention and treatment on induced rat's UC. To reveal the possible mechanism for treatment, we refer to a lot of literature information and Screened out some cytokines wich play an important role in the pathogenesis and healing of UC, included IL-1,TNF-αIL-10, which mainly regulate the immunity, SOD a cucial enzyme for antioxidant, COX-2 as inflammatory mediator, NF-icBp65 as transcription factor of Inflammatory cytokines and TGF-β1 which play an important role in regulating the repair of tissue injury. Observing the effect of ChangBiqing suppository on the expression of those cytokines in colonic mucosa of rats with ulcerative colitis to explore its therapeutic mechanism in immunology,anti-inflammatory and antioxidation, and provide some experimental basis and theoretical basis for ChangBiqing suppository in the treatment of UC. Results:The antigen expression of IL-1β,TNF-αin the model group were significantly higher(P<0.01) and expression of IL-10 was lower than the control group(P<0.01). Compared with the UC group, the expression of IL-1β,TNF-αin group B to E decreased markedly(P<0.01) and the expression of IL-10 increased significantly, and the change of cytokines were in a dose-dependent manner in group B to D; The antigen expression of SOD in the model group were significantly lower than the other groups(P<0.01), In contrast, no significant differences were observed among the four groups C, D,E and F; In modle rats with UC, the antigen expression of COX-2,NF-κBp65 and TGF-β1 were significantly higher(P<0.01) when compared with the control group.Compared with the UC group,the expression of COX-2,NF-κBp65 and TGF-β1 in group B to E decreased markedly(P<0.01) and in a dose-dependent manner in group B to D. In contrast, no significant differences in expression of COX-2 and NF-κBp65 were observed in group B and E as well as TGF-β1 in group C,D and E. Conclusions:ChangBiqing suppository can adjust immunization, anti-oxidation, anti-inflammatory and to promote tissue repair, this may be the mechanism for its treatment of UC.
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