The Anti-tumor Effects Of Radiotherapy Combined With Capecitabine On The Tumors Grown In Mice

Objective:Successfully constructed C57BL/6J mice Lewis lung cancer model, to explore the synergy of capecitabine and radiotherapy resistant tumors.Materials and methods:Take passage 14d of the Lewis lung tumor-bearing mice 2, hoping for were killed, in sterile conditions, stripping tumor tissue, tumor tissue with a surgical knife to cut in a sterile petri dish in 3-4mm3 size of tissue, uniform glass Preparation of cell suspension slurry grinding device, adjusting the density of 1.0×107个/ml. Right hind limb in each mouse subcutaneously lateral cell suspension 0.2ml. Planting when the tumor volume reached about 0.5cm3 randomly divided into 4 groups:control group, radiotherapy group, capecitabine group, radiotherapy+capecitabine groups and 12 in each group. Control group without any treatment; radiotherapy for local tumor irradiation 60Coy 5Gy/views x 4 times, once every other day; 60mg/kg/d capecitabine group were given capecitabine, dissolved in 200μ1 physiological saline,10 days since the beginning of radiotherapy Lian Yong; radiotherapy+capecitabine group use of radiation and capecitabine dosage as before. Every two days, the maximum length measurement of tumor diameter (A) and with the maximum width by the vertical (B), draw the growth curve of tumor volume. Mice were sacrificed 5 days after the end of the medication to take samples to compare each group tumor size, tumor tissue observed under light microscope pathological, immunohistochemical determination of tumor MVD values and VEGF-positive rate, compare the difference in each group.Results:1. Successfully established 48 mouse Lewis lung cancer models.2. Tumor volume:the main effect of radiotherapy was statistically significant factors, tumor volume in mice using radiation than the mouse does not use radiation therapy tumor volume (F= 7.924, P= 0.007); capecitabine factors also had a significant main effect the use of capecitabine in tumor volume than mice without the use of capecitabine in mice tumor volume (F= 7.008, P= 0.011). Radiotherapy+ capecitabine group were the minimum number of implanted tumor volume, that radiotherapy combined with capecitabine in the strongest anti-tumor effect. However, the interactive effect of two factors was not significant (F= 0.235, p= 0.630).3. HE staining, observed under light microscope to each treatment group than in the control group marked tumor necrosis, the radiation+capecitabine group of the most significant.4. Immunohistochemical staining:SABC to detect tumor tissue of each group the average MVD and VEGF expression positive rate:the use of radiotherapy in MVD and VEGF expression positive rate of less than non-radiotherapy group the positive rate of MVD and VEGF expression (F value of 397.91 and 695.33, P all<0.001);)The use of capecitabine group of MVD, VEGF expression was less than the non-use of capecitabine group of MVD and VEGF expression rate (F value of 301.44 and 498.06, P all<0.001); Both groups combined expression of MVD and VEGF positive rates of the minimum number that radiotherapy in combination with capecitabine in tumor tissue MVD and positive expression rate of VEGF strongest inhibition. There are interactions between capecitabine factors and radiotherapy factors (F values are 21.89 and 6.77, p<0.05).Conclusion:Radiotherapy combined with capecitabine in Lewis lung carcinoma in mice have synergistic anti-tumor effect, and can reduce tumor microvessel density and vascular endothelial growth factor expression.