Expression Of SIL-2R,IL-6 And CD4 In Thymoma With MG And Its Significance

Objective:Myasthenia gravis is an autoimmune disease mainly induced by acetylcholine receptor antibody on the postsynaptic membrane of the connecting parts of the neurological-muscles. The pathological changes may affect the AchR on the postsynaptic membrane of the connecting parts of the neurological-muscles. The main representation is that the skeletal muscle is easily fatigued though rests and the anti-cholinergic drugs the skeletal muscle can be partially recovered. It may become an autoimmune disease controlled by multiple mechanisms and many genes. Its athogenesis is connected with many factors such as the hereditary factors, antibody effects, cytokine effects, complements effects, thymus abnormalities and cellular immunity ect. The improper functioning of the immune system may be the important cause of the disease. However, at present, the initiating step causing immune response and the mechanism that makes T cells assist B cells to produce specific antibodies is still not clear. Thymus is the place where T cells mature. The thymus tissue of the patients with MG is rich in AchR sensitizing T cells, soluble interleukins 2 receptor (SIL-2R), interleukins 6(IL-6) and CD4+T cells which are important in the reaction of immune response. But there is still lack of research on their function in the process of producing disease of the patients with thymoma and myasthenia gravis. This research deals with the representation of the SIL-2R, IL-6 and CD4 in the thymoma with MG and discusses their function in the mechanism of producing disease.Methods:1. Collect the materials and the paraffin block of the 27 patients who experienced the thymoma operation in the Second Affiliated Hospital of Dalian Medical University. Among them, there are altogether 9 patients who had the thymoma and myasthenia gravis (MG group). Review and analyze the clinical data and study the relationship between the myasthenia gravis and the sex, age, MG clinical classification and the pathological classification of the thymoma.2. Take the method of immunohistochemistry to inspect the repre-sentation of SIL-2R, IL-6 and CD4 in the thymoma tissue.Results:1. The group of cases with thymoma and myasthenia gravis accounted for 33.33%(9/27)2. Thymoma and myasthenia gravis mostly happen among men and the ratio of men to women was two to one.3. The average age of men patients was lower than women patients.4. The pathological structure of thymoma in MG group was mostly type B1 and B2, covering 55.56%, the pathological structure of thymoma in non-MG group was mostly represented as type A, AB, B1 and C (83.37%), among them type C was in the majority, covering 33.33%.5. The representation of SIL-2R, IL-6 and CD4 in the thymoma tissue of the MG group was higher than that in the thymoma tissue of the non-MG group (p<0.05)Conclusion:1. The patients with both thymoma and myasthenia gravis were mostly male patients.2. Type B1 and B2 of thymoma was usually accompanied with myasthenia gravis.3. The expressions of SIL-2R, IL-6 and CD4 were high in the thymoma tissue of the patients with MG disease, suggesting SIL-2R, IL-6 and CD4 participated the pathogenesis of the thymoma and myasthenia gravis.