Preliminary Study Of Adjuvant Therapy Of Camel Milk On The Kidney Disease

To clarify the camel milk on kidney disease for adjuvant therapy, survey visits were undertaken in Mongolia Berger camel milk nursing home,and based on the human tracking test and animal experiment,preliminary study of the mitigation and improvement of camel milk on kidney disease.This study was divided into two sections:First section: human tracking survey test was undertaken to understand the camel milk on the impact of patients with kidney disease. 73 patients were randomly enrolled and divided into four age groups. Ten indexes of their urine, including specific gravity (SG), urinary pH, urinary protein(Pro), leucocyte (LEU), glucose(GLU), ketone(KET), urobilinogen (UBG), bilirubin (BIL), erythrocyte(ERY), nitrite(NIT) were detected and analyzed before and after nursing. Results:By comparing before and after the their convalescence of index of urine changes, it found that SG of 5 to 14years old patients group were significantly reduced(p<0.01), SG(p<0.01),PRO(p<0.05),GLU (p<0.05) of 15 to 29 years old patients were significantly reduced, SG(p<0.01) and GLU(p<0.05) of 30 to 44 years old patients were significantly reduced, SG (p<0.05) and pH(p<0.05) of 45 to 59 years old patients were significantly reduced. In addition, six index of urine,including leukocytes(LEU),ketones(KET),urobilinogen(UBG),nitrite(NIT),bilirubin(BIL),erythrocyte(ERY) before and after nursing has no significant change(p> 0.05). These results show that the camel milk may regulate some biochemical of the urine indicators of patients with kidney disease and play the role of adjuvant therapy. Second section: animal experiments was performed by establishing the chronic renal failure (CRF) model in rats and then used camel milk as administered intervention.Fifty Wistar rats were randomly divided into five groups: normal comparison group, pathology model group, Niaoduqing (a drug for curing CRF) intervention group, camel milk low dose group and high dose group with ten rats for each. In the morning four group rats were replicated CRF model by taking 200mg·kg-1·d-1 adenine for days and normal camparison group rats were administered physiological saline. At daily pm time, Niaoduqing intervention group, camel milk high and low dose group were respectively administered intervention niaoduqing solution and high or Low dose of camel milk, normal comparison group and pathological model group were administered with physiological saLine. Rats were sacrificed after 28 days administered intervention by high and low dose camel milk. The tested indexes included renal function (Scr, BUN), serum biochemical markers (NO, SOD, TP, Ca and P), 24 hour-urinary output, urinary protein, body weight, kidney weight and so on. In the end, make renal pathological section to observe the pathology changes for histopathology evaluation in kidney. Differences between groups were compared and evaluated. Results : It found that camel milk given group level of Scr,BUN was obviously lower than that in pathological model group(p<0.05), and level of Scr,BUN of each dose camel milk group have no significant difference with Niaoduqing intervention group, but higher than normal group(p<0.01). SOD activity of high dose and low dose camel milk group was higher than pathological model group, suggesting that camel milk had protective effect to some extent. levels of serum NO of high dose and low dose camel milk group had significantly decreased compared with pathology model group (p<0.01),and had no significant difference with niaoduqing intervention group. Serum total protein content of high dose camel milk group significantly higher than pathology model group(p<0.05), whiLe in low dose camel milk group also with higher level but had no significant difference. Blood caLcium and phosphorus content of high dose and low dose camel milk group were significantly higher calcium content(p<0.05) and lower phosphorus content(p<0.01) compared with model group, suggesting that camel milk played a regulatory role in body electroLyte disturbance. Compared with model group, urinary protein of high dose camel milk group had significantly decreased(p<0.05),while in low dose camel milk group also with lower level but had no significant difference(p>0.05). The pathology changes of kidney were observed under microscope showed camel milk have protection to nephron deterioration.Conclsion: Drinking camel milk can slow disease progression, which to some extent play a protective effect on kidney disease.