Salvia Miltiorrhiza Attenuates Ischemia-Reperfusion Induced Acute Lung Injury In Rats Following Hemorrhagic Shock

Objective:To explore mechanism of ischemia-reperfusion induced acute lung injury following hemorrhagic shock and that of salvia miltiorrhiza on lungs of hemorrhagic shock resuscitated rats.Method:Divide randomly average 30 healthy rats into normal control group(Group NC), hemorrhagic shock group(Group HS)and Salvia Miltiorrhiza group(Group SM). When the experiment finished, select the right lung to test the lung coefficients and make a pathological investigation of the lungs. We assessed the degree of pulmonary tissue injury by measuring the level of nitrate (NO3-)/nitrite (NO2-) levels, biochemically. We evaluated acute lung injury (ALI) by establishing pulmonary neutrophil sequestration and ALI scoring histopathologically. Pulmonary edema was estimated by using Evans blue dye extravasation and wet/dry ratios. Analyse the activity of myeloperoxidase (MPO), Monitor activity of the glutathione peroxidase (GSH-PX), and the activity of catalase (CAT).Result:1.To be compared with group NC, serum content, and NO content in lungs in group of SM and HS increase drastically. The NO content of group SM was lower than that of group HS (P<0.01).2.Lung coefficients decreased after treatment with SM and pathological changes of lungs apparently weaken than group HS. The group of SM treatment significantly reduced the activities of oxidative enzymes on MPO(P <0.01).3.The activities of enzymes such as CAT and GSH-PX of group SM and HS compared with group NC, and the group of SM treatment significantly increased the activities of enzymes on CAT and GSH-PX(P<0.01). Nitrate/nitrite levels in the lung tissues were decreased by SM compared with group HS.4.Pulmonary neutrophil sequestration and ALI scores were decreased significantly with SM administration (P <0 .01).Conclusion:1.This study shows that the pulmonary parenchyma is very vulnerable to free oxygen radicals induced by I/R, which cause oxidative damage. SM treatment significantly attenuate the lung reperfusion injury after hemorrhagic shock resuscitate.2.The inhibition of oxidative enzymes can be explained mainly by the anti-inflammatory effects to participate in the course of lung damnification, and SM plays a good and protective role in lung damnification on anti-inflammatory action and anti-oxidative stress. At meanwhile, the contents of NO in the lung tissue of I/R are decreased after SM treatments, which indicat that SM maybe inhibit the activity of iNOS.