Immune Responses Induced By Mucosal Immunization With Group B Streptococcus Conjugate Vaccines

Objectives To observe the effect to the immunogenicity of different mucosal administration routes of GBS-CPS-TT,GBS-CPS-DT conjugate vaccines.Methods NIH mice were divided into 8 groups,30 mice per group. Mice were immunized respectively with GBS-CPS-TT,GBS-CPS-DT conjugate vaccines 3 times with an interval of 2 weeks by intranasal or intragastric or hypodermic injection route. The amounts of IgG against GBS were measured by ELISA.Results The levels of IgG in serum were significantly increased after intranasal immunization. The levels of IgG of the group of 10μg intranasal immunization was significantly higher than the group of 5μg intranasal immunization(P<0.01). It was also shown that immunological memory and boosted response could be elicited by GBS-TT or GBS-DT conjugate vaccines. TT or DT shows no difference to be the carrier to make GBS-protein conjugate vaccines (P>0.05).The levels of IgG of the group of the control group can not be detected.Conclusions GBS-CPS-TT,GBS-CPS-DT conjugate vaccines can induce system immunity reaction by intranasal immunization, the higher immunizing dose may achieve the better immunizing results and this method of immunization appears to be a safe and effective immunization route. The effect of immune responses induced by mucosal immunization needs more study.