Relation Between Transient Receptor Potential Vanilloid-1 Gene Polymorphism And Irritable Bowel Syndrome

Obective:Irritable bowel syndrome is one of the most common functional gastrointestinal disorders which the pathogenesis is not yet clear. It is characterized by chronic and recurrent abdominal pain or discomfort along with altered bowel function. Genetic factors alter the susceptibility to IBS of individuals. Our objective is to investigate the relationship between transient receptor potential vanilloid-1 (TRPV1) gene polymorphism and irritable bowel syndrome (IBS) and its visceral hyperalgesia (VHL).Methods:Polymerase chain reaction(PCR)-Restriction fragment length polymorphism (RFLP) and direct sequence was used to comparatively analyze the polymorphism of TRPV1 gene with IBS (n=73) fulfilling Rome III criteria and healthy control(n=65). PC Polygraf ID gastrointestinal function testing equipment and 8-chaanel perfusion with a balloon-type piezometer was Used to test the IBS (n=31) and healthy controls'(n=16)anorectal feeling and the first sensation volume(FSV), defecating sensation volume(DSV), maximum tolerable volum (MTV) and painful sensation volume(PSV) was recorded.Result:We found three genotypes in the case and controls that they were CC, CT and TT. The frequencies of CC genotype and C allele in IBS patients were significantly higher than those in the controls (genotype:X2=6.858,P=0.032; allele:X2=4.174, P=0.041). Significant difference was found in TRPV1 gene polymorphism between IBS and control (P<0.05). FSV(24.71ml±5.52ml vs 32.86ml±5.67ml P=0.019), DSV(64.29ml±9.75ml vs 80.01 ml±14.41 ml,P=0.03), MTV (84.57ml±9.07ml vs 133.57ml±9.88ml,P<0.001), PSV (143.57ml±9.89ml vs 231.42ml±12.49ml,P<0.001) of IBS are much lower than healthy control(P<0.05).Conclusion:VHL is one of the pathophysiology characters of IBS and play an important role in IBS. Polymorphism of TRPV1 gene is associated with IBS. TRPV1 is expected to be a novel therapeutic target for IBS.