Abstract Objective:To investigate the biotransformation of artemisinic acid and dihydroartemisinic acid by cell suspension culture of Cephalotaxus fortunei Hook. f. and transgenic crown gall of Panax quinquefolium L, generated three transformed Products and a secondary metabolite.Methods:Plant tissue culture technology was employed. Transformed products were detected by TLC and HPLC, isolated and purified by the column chromatography, recrystallization, Sephadex LH20, ODS etc. Their physico-chemical properties were checked up. Their structures were elucidated by 1H-NMR, 13C-NMR and MS. The optimal co-culture times of the products were determined by HPLC.Results:The cell suspension culture of Cephalotaxus fortunei Hook. f. transformed the artemisinic acid and dihydroartemisinic acid to three Products, transformed artemisinic acid to one Product:3a-hydroxyartemisinic acid. After co-cultured for 2 days, the mole conversion ratio of artemisinic acid reached the highest (8.42%) in cell suspension culture of Cephalotaxus fortunei Hook. f. The cell suspension culture of Cephalotaxus fortunei Hook. f. transformed dihydroartemisinic acid to two Products: 15-hydroxy dihydroartemisinic acid and 15-hydroxy dihydroartemisinic acidβ-D-glucopyranosyl ester. The conversion rate of SQQJ-3 in the cultures reached the maximum (6.02%) in 2nd day, and conversion rate of SQQJ-5 in the cultures reached the maximum (4.95%) in 3rd day. The transgenic crown gall of Panax quinquefolium L transformed artemisinic acid, generated a secondary metabolite:12,13-epoxy-11-hydroxy-9-octadecenoic acid.
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