The Impact Of Progesterone Receptor Isoforms Expression To Invasion Capacity And SULT1E1 In Endometrial Cancer Cell

ObjectiveTo explore the function of progesterone receptor to the invasion of Ishikawa adenocarcinoma cells and the relation between SULT1E1 and progesterone receptor isoforms in the cells.MethodsTransfacted progesterone receptor isoforms plasmid into Ishikawa endometrial cancer cells,and observed the change of invasion of Ishikawa endometrial cancer cells and mRNA and protein of SULT1E1.Results1. Plasmid restriction analysis appraisal with plasmid structure.2. The relative content of protein expression of hPRA in hPRA transfected cells is 387.855±3.2032, the relative content of protein expression of hPRA was significantly increased compared with Control group(189.905±2.8634), empty vector transfected group(198.03±0.4384) (p＜0.05), The relative content of protein expression of hPRB in hPRB transfected cells is 440.55±5.445, the relative content of protein expression of hPRB was significantly increased compared with Control group(65.996±3.1734), empty vector transfected group(71.455±0.8132) (p＜0.05), The relative content of protein expression of hPRA in hPRA+hPRB cotransfected cells is 286.59±5.1477, the relative content of protein expression of hPRA was significantly increased compared with Control group (84.888±0.328), empty vector transfected group(87.315±1.393) (p＜0.05), The relative content of protein expression of hPRB in hPRA+ hPRB cotransfected cells is 167.15±2.602, the relative content of protein expression of hPRA was significantly increased compared with Control group (80.832±1.962), empty vector transfected group(81.715±0.9122) (p＜0.05).3. hPRB cells decrease the invasion of the cell (p＜0.05);4. Control group, empty vector transfected group, hPRA transfection group, hPRB transfection group, hPRA+hPRB group SULT1E1 mRNA relative expression levels were 1,0.6263,0.6690,5.0982,0.5116, the SULT1E1 mRNA relative expression levels of hPRB transfected group was significantly increased compared with Control group, empty vector transfected group (p＜0.05), hPRA+ hPRB co-transfected group was significantly decreased compared with and hPRB transfection group 5. Control group, empty vector transfected group, hPRA transfection group relative expression of SULT1E1 protein were 139.51±5.2609,152.92±3.3375,153.67±6.1943, the differences were not statistically significant (p＞0.05),but hPRB transfection group relative expression of SULT1E1 protein was 652.59±6.017, was significantly increased compared with Control group, empty vector transfected group (p＜0.05); hPRA+hPRB co-transfected group relative expression of SULT1E1 protein was 164.11±2.4607, the differences were not statistically significant compared with Control group, empty vector transfected group (p＞0.05); co-transfected group SULT1E1 protein expression was significantly decreased, compared with hPRB transfection group (p＜0.05).Conclusions1. hPRB can decrease the invasion of the Ishikawa cell; 2. Transfection of the progesterone receptor isoforms hPRB in Ishikawa cells can upregulate expression of mRNA and proteinexpression of mRNA and protein 3.The function of hPRB was inhibited by hPRA.