Expression Of The MiR-17-92 Polycistron In Primary Hepatocellular Carcinoma And Its Clinical Significance

ObjectivemiR-17-92 gene cluster promote cell cycle from G1 phase to S phase transition and cell proliferation, and inhibit apoptosis by inhibiting expression of the tumor suppressor gene PTEN, BIM and the cell cycle regulatory genes E2F1, E2F2, E2F3. The expression of miR-17-92 in many tumors may be related to the occurrence of cancer. To explore the relationship between the miR-17-92 expression in hepatocellular carcinoma and tumor genesis, development as well as Pathological characteristics, we detected the miR-17-92 expression of hepatocellular carcinoma and adjacent tissues in 30 cases, and analyzed the relationship between abnomal expression of miR-17-92 and Pathological characteristics of tumor such as size, differentiation, clinical stage, liver cirrosis,envelope, serum AFP concentration so on.MethodsWe extracted the total RNA of hepatocellular carcinoma and adjacent tissues in 30 patients with hepatocellular carcinoma and the total RNA of normal liver tissue adjacent to hepatic hemangioma in 5 patients. Then we used Real time RT-PCR to detect miR-17-92 expression in these tissues, and analyzed the relationship between miR-17-92 expression and Pathological characteristics of tumor such as size, differentiation, clinical stage, liver cirrosis,envelope, serum AFP concentration by single-factor variance analysis. ResultsCompared with the adjacent tissues, the expression of miR-17-92 increased in 14 cases of primary hepatocellular carcinoma, accounting for 46.67%(14/30); and decreased in 16 cases, accounting for 53.33%(16/30).Compared with hepatocellular carcinoma adjacent tissues,there is no significant difference (P>0.05). Compared with normal liver tissue, the expression of miR-17-92 increased in 25 cases of primary hepatocellular carcinoma, accounting for 83.33%(25/30); and decreased in 5 cases, accounting for 16.67%(5/30). The expression of miR-17-92 in hepatocellular carcinoma was significantly higher than normal liver tissue (P<0.01). miR-17-92 expression up-regulation was related to differentiation,the clinical stage and liver cirrosis (P<0.05), but had no significant correlation with the size, envelope, serum AFP concentration, hepatitis B (P> 0.05).ConclusionUp-regulation of miR-17-92 took part in the genesis and development of hepatocellular carcinoma.The abnomal expression of miR-17-92 was related to the tumor differentiation,clincal stage and liver cirrosis in hepatocellular carcinoma.