| ObjectiveTo investigate the proportion of peripheral blood CD4+CD25+Foxp3+ regulatory T cells in pancreatic carcinoma patients, the dynamic presence of CD4+CD25+Foxp3+ regulatory T cells before and after pancreatic carcinoma resection was also analyzed. Analyze the roles of CD4+CD25+Foxp3+ regulatory T cells in the pancreatic carcinoma immune tolerance.MethodsCompare the proportions of peripheral blood CD4+CD25+Foxp3+ regulatory T cells in pancreatic carcinoma patients and healthy donors by flow cytometric analysis. Foxp3 and CD4+/CD8+ ratios were also detected, and compared preoperative and postoperative.ResultsTreg were increased in pancreatic carcinoma patients compared with those in healthy control.Additionally, the prevalence was reduced significantly after resections, which was most obviously on the 3rd day after operations, this tendency was almost equal to that of CA19-9.These CD4+CD25+ Tregs had high expression of Foxp3.There were an decreased ratios of CD4+/CD8+, which further declined after the operations, the levels began to decease in the following week when the levels were equal to those in healthy donors.ConclutionCD4 CD25+Foxp3+ regulatory T cells play a central role in self-tolerance of pancreatic carcinoma. Pancreatic carcinoma resection may be helpful for the recovery of anti-tumor immunity. The perioperative period of pancreatic carcinoma patients may be a beneficial window-phase for immune intervention and regulatory T cells may be served as a target cell. |
