The Expression Of FXR,MDR3 And MRP In Liver Cancer Cell Lines And Tumor Tissues

Objective:The main cause of death in patients with hepatocarcinoma is tumor recurrence and metastasis. It is well known that nuclear receptor FXR (farnesoid X receptor) is playing an important role in liver carcinogenesis. However, the effect of FXR on hepatocarcinoma metastasis was seldom reported. In this study, the FXR and its downstream gene such as MDR3, MRP were detected in liver cancer cell lines with different metastatic potentiality and tumor tissues from the patients with primary liver cancer. The influencing factors of liver carcinogenesis were further analyzed in order to find new biological therapeutic targets in liver cancers.Methods:The expression of FXR, MDR3, and MRP in hepatocellular carcinoma cell lines with high and low metastatic potentiality was detected by fluorescence quantitative real time PCR. Then, the tumor tissues, perineoplasm tissues and normal tissues from 28 cases of patients with primary liver cancer were collected for the detection of the expression of FXR, MDR3, and MRP by quantitative real time PCR. The serum combined total bile acid (TBA), total bilirubin (TB) and conjugated bilirubin (CB) changes of the patients were analyzed by biochemistry laboratory. Results:FXR, MDR3, and MRP expression were significantly lower in the high metastatic potentiality hepatocellular carcinoma cell lines than the low one (p<0.05) And the expression of FXR, MDR3, and MRP in tumor tissues were lower than perineoplasm tissue in tumor patients(FXR,MDR3 group p>0.05, MRP group p<0.05). The TBA, TB, CB were elevated in 33.3%,11.1%and 44.4% patients with high invasive ability liver cancer, while the TBA, TB, CB were elevated in 44.4%,66.7% and 50.0%patients respectively in the low invasive ability group. In addition, the expression of FXR, MRP were significantly higher (p<0.05) and MDR3 was significantly lower (p<0.05) in high invasive ability tumor tissues than that of low invasive ability group.Conclusions:There are different expression of FXR, MDR3, and MRP in the high and low metastatic potentiality of hepatocellular carcinoma cell lines and tumor tissue in patients with liver cancer, which indicating that these genes might be related to the tumor metastatic potentiality.