| Myocardial infarction is the most common cause of death. Rapid establishment of reperfusion after coronary occlusion is the main therapeutic target of current cardiologic therapy. However, ischemic reperfusion may induce organ dysfunction, which may be detectable as arrhythmias, myocardial stunning, or microcirculatory flow disturbances such as low or no reflow. Postconditioning is one of the most effective methods in treatment on ischemia/Reperfusion injury in recent years. Postconditioning is that short periods of ischemia and reperfusion, applied during early reperfusion, also reduce myocardial injury. In recent years, application of drugs to simulate the role of postconditioning, that clinicians increasingly affirmed.The gross saponins of Tribulus terrestris (GSTT) was one of the effeetive group ingredients which was one of extracted from the whole Plant of Caltrop. It is a mixture of over 10 kinds of Saponins, mainly steroid Saponins.It mainly includes furan-strol and spiral-strol.It has been used in clinical cardiovascular and cerebrovascular diseases. A large number of basic and clinical studies confirm: GSTT has anti-atherosclerosis, expand coronary artery, improve cardiac function, improve blood rheology, lower plasma cholesterol and triglycerides, anti-myocardial ischemia, hypoxia and other effects. In addition, at present, Application of GSTT to simulate the role of postconditioning that is still less reporting.Objective: This study is to observe Myocardial ischemia-reperfusion injury model prepared by using the method of ligating the left anterior descending coronary artery of the rat's heart to explore whether GSTT postconditioning had a protective effect on myocardial ischemia-reperfusion injury in rats. Methods: Rats were randomly divided into sham operation group (Sham), ischemia- reperfusion group (I/R), ischemic postconditioning group (IpostC), positive drug group nicorandil, GSTT 100, 30, 10 mg?kg-1 group. Levels of lactate dehydrogenase (LDH), 2-amino diphenyl methane (MDA) and superoxide dismutase (SOD) in serum were determinated using spectrophotometry. TNFαand IL-6 levels were determinated with ELISA. Histopathological changes of myocardial damage was observed with optical microscope and apoptosis of myocardial tissue was detected by TUNEL. Results: Compared with model group, levels of LDH, MDA, TNF-αand IL-6 in GSTT 100, 30 mg?kg-1 group were significantly lower, SOD activity was significantly higher (P< 0.01), the number of apoptotic cells was significantly reduced (P<0.01), and myocardial morphology was significantly improved. Conclusion: At the same time of reperfusion, intraperitoneal injection of GSTT as pharmacological postconditioning on myocardial ischemia-reperfusion injury has a protective effect by reducing free radicals and inhibiting cell apoptosis. |
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