| Essential hypertension (EH) is a complex disease with multi-gene and multi-environmental trait, accounting for more than 90% in hypertension. Recently, the prevalence of hypertension showed a increasing trend all over the world. It has forecasted that, by 2025 there will be 1.58 billion hypertension patients in the world. According to the epidemiological survey in the 10th Five-Year Plan, the standardized prevalence of hypertension in China has reached 24.4%. There are more than 200 million patients with hypertension in China. It has estimated that approximately one million premature deaths had relationship with hypertension every year. The prevention and control of hypertension is a very formidable task in China. Therfore, while the important significance of prevention and treatment of hypertension are stressed, the etiologic research in hypertension is also a hot topics.Hypertension has the characteristics of highly genetic heterogeneity, delayed dominant and complex pathogenesis. Increasing evidence shows that many patients with essential hypertension showed a cellular and humoral immune disorder, so the conclusion has been drawed that HLA antigen might play an important role in blood pressure regulation. Single nucleotide polymorphisms have been widely used of screening the susceptibility gene in recent years. However, There are relatively few reports about the relationship between the SNPs of HLA-DRB1 and essential hypertension.ObjectiveIn order to explore the association between HLA-DRB1 gene polymorphism and essential hypertension in the terms of gene level, provide new clues for the genetic mechanism of hypertension, and lay the theoretical basis for clinical treatment of hypertension, rs2308765, rs9269186 and rs3135388 will be slelected to study the relationship between HLA-DRB1 and essential hypertension. And the internal environmental factors of hypertension will be analyzed for the purpose of further exploring the factors affecting the development of hypertension, and providing a reasonable reference for the prevention and control measures.MethodsThe SNPs of HLA-DRB1 were detected by polymerase chain reaction-ligase detection reaction technique in samples from 161 patients with essential hypertension and 167 healthy controls. The Genemapper software was used for the analysis of SNPs data and gene types.Epidata 3.0 and SPSS13.0 softwares were used to perform analysis. Mean and standard deviance was used to descripe continuous variables. Rate and ratio were used to describe categorical data. T test or Rank test was used to test the differences of continuous variables between case and control.χ2 or Fisher's exact test were used to test the differences of categorical data between different groups. The goodness-of-fitχ2 test was used for Hardy-Weinberg equilibrium test in case and control. The differences of genetype and allele frequencies between case and control were test byχ2 test. T test or Rank test was used for univariate analysis on the enviromental factors. The multivariable non-conditional logistic regression model was used for the multivariate analysis and the interaction analysis between gene polymorphisms and enviromental factors.Results1. 308 samples were investigated, which were divided into two groups. 161 patients belonged to case group, while 147 samples belonged to control group. The essential characteristics of objectives included the following aspects: The average age was 64.6±10.2, and the 60～69 age-group had the highest constituent ratio(35.1%), While the proportion of less than 50 years was the lowest; Male took the most part of samples(92.8%); Most of them were highly educated, about 82.4% were Junior College or College; Han was the main nationality(92.2%). The differences in age, gender, nationality and cultural status were not found between case and control. 2. The results by PCR-LDR indicadated that, Only G/G and G/T genetypes of rs2308765 expressed ; C/C, G/G and C/G genetypes of rs9269186 were found in the research; C/C and T/T genetypes of rs3135388 were found.3. The genetypes and alleles distributions of rs2308765, rs9269186 and rs3135388 between case and control were accorded with Hardy-Weinberg equilibrium law (P>0.05).4. The differences of genetype and allele distributions in rs2308765 between case and control were significant (P<0.05). When the mutation was taken as an exposed factor, The OR values of genetype and alleles respectively were 2.774 and 2.675, and the 95% confidence interval of OR didn't include 1.5. The significant differences of genetype and allele distributions in rs9269186 and rs3135388 were not found.6. Between case and control, the low-density lipoprotein showed an edge significace(P=0.059), and BMI, waist circuit, blood sugar, total cholesterol, triglycerides and high-density lipoprotein showed significant differences(P<0.05). The value of BMI, waist circuit, blood sugar, total cholesterol, triglycerides and low-density lipoprotein in case were higher than in control, while the high density lipoprotein value was lower than in control.7. The multivariable logistic regression showed that high BMI and high triglycerides were risk factors of essential hypertension.8. The interation annalysis indicated that there was a negtive plus interation between rs2308765 polymorphisms and triglycerides.Conclusions1. There was a certain correlation between rs2308765 polymorphisms and essential hypertension, and G/T mutation might be the risk factor of essential hypertension.2. rs9269186 and rs3135388 polymorphisms did not associate with essential hypertension.3. The value of BMI, waist circuit, blood sugar, total cholesterol, triglycerides and low-density lipoprotein in case were higher than in control, while the high density lipoprotein value was lower than in control.4. High BMI and high triglycerides were the risk factors of essential hypertension.5. There was a negtive plus interation between rs2308765 polymorphisms and triglycerides. |
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