| [Objective] In this study, rats were fed with Iodine excess and monophagia diet, the establishment of the rat models of Iodine excess and monophagia diet, using of the measuring of coagulation index, the determination of plasma HCY, immunohistochemistry results of rat inferior vena cava and HE slice of rat inferior vena cava, to observe the effects of different concentrations of Iodine on rat blood coagulation function and the injuries of excess Iodine on the rat vascular endothelial cells in the inferior vena cava, and to study the mechanism of the excess Iodine on rat endothelial cells and blood coagulation.[Material and Methods]1. Experimental animals and groups:selecting of approximately 1 month after weaning, weighing 100-120g outbreeding closed colony of Wistar rats (purchased from the Animal Experimental Center, Shandong University, is a SPF/VAF-class animals), according to weight and sex, the rat were randomly divided into 8 groups, the order as follows:①Normal control group(NI):feed normal diet and drink normal water;②10HI group:drink 10 multiple excess Iodine water;③50HI group:drink 50 multiple excess Iodine water;④100HI group:drink 100 multiple excess Iodine water;⑤Monophagia control group(BNI):feed monophagia diet and drink normal water.⑥B10HI group:drink 10 multiple excess Iodine water;⑦B50HI group: drink 50 multiple excess Iodine water;⑧B100HI group:drink 100 multiple excess Iodine water. To weigh rats'body weights weekly and collect urine to observe their Iodine metabolism at 2,4 and 6 months later. N=8. 2. Records of body weight data of the rats:The above animals weekly weigh in and record. Feeding for 6 months.3. The coagulation indexes were measured:got the rats'blood from eyeball vein by enesthetize with ethylether. To test the blood coagulation index as follows:PT-Time, PT-INR, APTT, TT, Fbg-Time, Fbg-C, AT3:A, DD-dimer by Sysmex CA7000 automated blood coagulation analyzer.4. Measuring HCY of Rats in each group5. HE staining of inferior vena cava of the rats:accessing 10% formalin fixed the inferior vena cava in rats, routine paraffin embedding, slicing, conventional dewaxing with xylene, the various levels of ethanol to water, hematoxylin 5min, tap water rinsing, Hydrochloric acid alcoholdiffere-ntiation 30s. Water immersion 15min. Routine dehydration, clearing, mounting, observing changes in endothelial cells.6. Immunohistochemical chemistry of the inferior vena cava in rats:tissue section through the dewaxing and hydration, antigen repaired, PBS washing, dropping the primary antibody, second antibody, conducting DAB color, observing ET-1, ICAM-1 in the inferior vena cava blood vessel endothelial cells.[Results]1. Body weight changes in ratsInterventing 2,4,6 months, comparing to NI group, the rats of feeding the normal and excess iodine group was no significant difference, until the first 6 months, comparing to NI group, the body weight of the Monop-hagia control group (BNI) a statistical difference; interventing 2,4,6 months, comparing to NI group, the body weight of the Monophagia control group (BNI) were significantly lower than NI group2. The coagulation indexes in ratsComparing with normal control rats (NI), The normal diet and drinking excess Iodine water group The numerical value of PT, PT-INR, Fbg-time, TT, APTT, AT3:A decreased significantly and the numbers of Fbg-C increased significantly in NC+10I group comparing to NC group (PT,PT-INR,Fbg-time:p<0.05; TT,APTT,Fbg-C:p<0.01). The numerical value of PT-time,PT-INR,Fbg-time,TT, APTT increased significantly and the the numerical value of Fbg-C decreased significantly in 501 and 1001 groups comparing to the normal control group (except Fbg-Time,TT,Fbg-c in 50HI p<0.05, the other blood coagulation index in 50HI and 100HI p<0.01).The Monophagia control groups are Compared to the normal control groups, the numerical value of PT-Time, PT-INR, Fbg-Time, APTT, TT and AT3% of the B10HI rats decrease, The numerical value of fibrinogen concentration (Fbg-c) was increased (except APTT p<0.01, the remaining is non-statistically significant) and The numerical value of PT-Time, PT-INR, Fbg-Time, TT and APTT of BNI, B50HI and B100HI rats increased, the numerical value of fibrinogen concentration (Fbg-c) values decreased. (Except TT of B50HI group was not non-statistically significant, the other values P<0.01). The Monophagia control groups comparing with the normal control group feeding with excess iodine, blood coagulation degraded.3. The Results of HCYAs the iodine concentration gradually increasing, HCY levels gradually increased. With the normal control group (NI) compared to,10HI group, 50HI group,100HI group, B10HI group, B50HI group, B100HI group gradually increased HCY levels were significantly different (except 10HI group, the rest of the group p<0.01) The excess Iodine group comparing to normal control rats, homocysteine (HCY) levels of 50HI and 100HI group significantly increased.4. Picture shows endothelial cells of inferior vena cava in excess Iodine group rats changed. Along with the iodine concentration increasing, endothelial cells of rat inferior vena cava prolifered. The Monophagia control group is more significant than the normal control group.5. Immunohistochemistry results in inferior vena cava of the rats showed that in the endothelial cells of inferior vena cava in the excess iodine group, ET, ICAM-1 Significantly increased. Comparing to the normal control group, the Monophagia control group is more significant, the normal control group expressed weakly.[Conclusions]1. These findings suggest that excess Iodine played a dual role on coagulation function, it can promote and reduce the coagulation function, the function is related with iodine dose. Appropriate amount of iodine promote coagulation function, but along with the iodine concentrations increasing, the coagulation function reduced. the Monophagia control can reduce blood coagulation in rats.2. Morphologic study found that excessive iodine can lead to inferior vena cava endothelial hyperplasia, thickening, and endothelial lower extracellular matrix increasing; ET, ICAM-1 expression was significant, and are related with the dose of iodine increasing, the Monophagia control is more significant, suggesting that in the excess iodine animal models, by stimulating endothelial cell injury and proliferation, caused fibrous tissue hyperplasia local in the inferior vena cava.3. In the animal model, excess iodine can lead to plasma homocysteine elevating, especially the Monophagia control, indicating the detection of homocysteine could be used as indicators of endothelial injury.
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