JNK2 Early Retinopathy In Diabetic Mice And The Related Effect Of The Expression

Objective:Through observing JNK2 to mice in the early course of diabetic retinopathy and changes in the expression of retinal ganglion cells in apoptosis, Discussing JNK2 in diabetic retinopathy (DR) mechanism of the early, and to further explore the pathogenesis of diabetic mechanism and provide theoretical basis for early treatment.Methods:C57BL/6 male mice were randomly divided into experimental and control groups, the experimental group were administered intraperitoneally with 1% STZ-induced diabetes model, the control group were injected with doses of sodium citrate buffer, model by made success insist 2,4,8 weeks after operation, taking out eye of the mices and left eye retinal extract for RT-real-time PCR, detection of JNK2 mRNA expression at different times changes in the eye right eye out, HE staining and TUNEL methods, respectively, in the period of retinal morphology and retinal ganglion cell apoptosis.Results:(1) JNK2 expression in the retina of DM:The normal group of mice in the expression of no significant difference in diabetic mice 2 weeks with the same expression of normal mice JNK2 was no significant difference (t=-0.109, p= 0.915＞0.05); January group of diabetic mice than the same period in normal JNK2 expression increased (p= 0.002＜0.05); 2 month group were significantly increased expression of JNK2 (p= 0.000＜0.01)(2) The rate of ganglion cells:ganglion cells of normal mice have a small amount of apoptosis, and no significant difference among the groups, diabetes 2 weeks of ganglion cells was not significant (between groups p= 0.086＞0.05); diabetes 4 weeks, retinal ganglion cell layer of apoptotic cells can be found(between groups p= 0.000＜0.01); diabetes 8 weeks, ganglion cell layer of apoptotic cells increased, inner nuclear layer and outer nuclear layer is also visible apoptotic cells. (between groups p= 0.000＜0.01)(3) HE staining and ganglion cell counts:normal layered retinal clear, RGCS arranged in neat rows, the inner plexiform layer staining is uniform, inner nuclear layer and outer nuclear layer cells were arranged in order, as the cone cells, rod cells lined; 2 months group and the normal layers of the retina of diabetic mice was no significant change in the experimental group of mice 4 weeks slightly disordered layered retina, normal morphology, but more sparse; DM 2 month group reduce the number of ganglion cells in the more obvious nerve fiber layer and inner plexiform layer of thin, inner nuclear layer cells arranged irregularly, outer plexiform layer is thinning, fuzzy external membrane, rod cell layer disorder. Ganglion cells 2 weeks of diabetes compared with no significant difference between the normal group (between groups p= 0.137＞0.05), diabetes in January the number of ganglion cells in mice began to become less (between groups p= 0.000＞0.05); group two months to further reduce the number of ganglion cells (between groups p= 0.000＞0.05).Conclusion:(1) Diabetic retinopathy is a variety of factors, many ways to the complex process of co-regulation.(2) JNK2 in early diabetic retinopathy may be important regulatory factor, and its expression is closely related with the DM of course.(3) JNK2 and the expression of retinal ganglion cells apoptosis in nerve cells was positively correlated.(4) JNK2 inhibitors may be a new method during early treatment of diabetic...