Effect Of Gonadotropin Releasing Hormone Analogues On Apoptosis Of Cultured Adenomyosis Endometrial Cells

Objective1. To investigate the effects of gonadotropin releasing hormone analogues (GnRHa) on apoptosis of cultured adenomyosis(AM) endometrial cells.2. To determine the roles of abnormality of apoptosis of AM endometrial cell in the pathogenesis of adenomyosis and provide new approach and experimental evidences for the clinical treatment of adenomyosis.Materials and methods1. SamplesEutopic endometrial cells from 32 women who received hysterectomy for AM(study group) and 20 women who received ovarian cyst surgery for benigh ovarian epithelial tumor(control group) of gynaecology department in our hospital were cultrued in vitro.2. MethodsDetect the apoptotic ratio by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and flow cytometry(AnnexinV-FITC/PI) before and after using GnRHa.3. Statistics analysisData were recorded as (x|-)±S. The results were analyzed by one-factor analysis of variance (ANOVA) with SPSS13.0 statistical software.The results were analyzed by LSD-t if they have homogeneity of variance,by Dunnett-t if they have not.Significance was set at P<0.05.Results1. Cells in two groups showed cell shrinkage,floating mucleus concentration,nuclear fragmentation and typical apoptotic bodies.2. The apoptotic ratio of cultured AM endometrial cells without GnRHa was lower than that in control group and the apoptotic ratio increased with the prolongation of time in two guoups(P<0.01).3. After GnRHa addition,each group showed higher apoptotic ratio and a trend towards higher apoptotic ratio linked to advanced concentration.Furthermor, apoptotic ratio in study group was significantly higher than in control group at the same time point and same concentration(P<0.01).Conclusions1. The abnormality of apoptotic ratio of AM endometrial cells may be associated with the of the AM pathogenesis.2. GnRHa may increase the apoptotic ratio of cultured AM endometrial cell by autocrine or paracrine,and provides the experimental and theoretical evidence for GnRHa clinical application.