The Correlation Study Among The Nucleotide Polymorphisms Of COX-2, PARP-1 And The Su Sceptibility To Gastric Cancer In Hui Ethnic Group

Objective To explore the correlation of nucleotide polymorphisms in cyclooxygenase-2(COX-2) -765G＞C,poly(ADP-ribose)polymerase-1 (PARP-1) Va1762Ala(T2444C) and the susceptibility to gastric cancer in Hui ethnic group.Methods All investigated objects were divided into 3 groups:100 gastric cancer patients,102 precancerous lesion patients and 105 control subjects were enrolled.Polymerase chain reaction-restrieted fragments length polymorphism(PCR-RFLP) method was used to defect genotypes of -765G＞C and 2444 T/C polymorphisms.ELASA method was used to identify IgG to H.pylori.The results were analyzed by SPSS13.0.Result COX-2-765GC+CC genotypes were overrepresented in GC(the gastric cancer group)(43.0%) compared with controls(27.6%)(OR,1.977, 95%C1:1.104～3.541,P=0.021).PARP-12444TC+CC genotypes were overrepresented in GC(36.0%) compared with controls(22.8%)(OR,1.898; 95%Cl,1.030～3.500,P=0.039).Stratification analysis showed that:among group with positive HP infection,-765GC+CC carriers could increased the susceptibility to gastric cancer compared with -765GG carriers(OR:2.121, 95%CI:1.000～4.498,P=0.048)and 2444TC+CC carriers could increased the susceptibility to gastric cancer compared with 2444TT carriers(OR: 2.270,95%Cl:1.023～5.037,P=0.042).Interaction analysis showed that: -765GC+CC carriers with Positive family history of GC had 3.738 fold increased risk for developing gastric cancer compared with -765GG carriers with negative family history of GC(OR:3.738,95%Cl:1.451～9.628, P=0.005);-765 GC+CC carriers with eating pickle vegetables had 5.038 fold increased risk for developing gastric cancer compared with -765GG carriers with not- eating pickle vegetables(OR:5.038,95%Cl:2.261～11.224, P=0.000);2444TC+CC carriers with eating pickle vegetables had 3.408 fold increased risk for developing gastric cancer compared with 2444TT carriers with not-eating pickle vegetables(OR:3.408,95%Cl:1.505～7.714, P=0.003).-765 GC+CC carriers with positive H.pylori infection had 3.520 fold increased risk for developing gastric cancer compared with -765 GG carriers with negative H.pylori infection(OR:3.520,95%Cl.1.579～7.850,P=0.002); 2444TC+CC carriers with positive H.pylori infection had 2.889 fold increased risk for developing gastric cancer compared with 2444TT carriers with negative Hp infection(OR:2.889,95%Cl,1.265～6.598,P=0.010).The subjects simultaneously carrying -765 GC+CC and 2444TC+CC genotypes had 3.771 fold increased risk for developing gastric cancer compared with subjects simultaneously carryng -765 GG and 2444TT(OR,3.771;95%Cl, 1.429-9.947;P=0.021).Conclusion COX-2-765G＞C and PARP-1Va1762Ala(2444T/C) contributed to the increased susceptibility to gastric cancer in Hui ethnic group in Linxia City,Gansu Province.Multiplicative interactions lied in between COX-2-765 GC+CC and positive family history of GC,-765 GC+CC,2444TC+CC and eating pickle vegetables,-765 GC+CC,2444TC+CC and H.pylori infection,-765GC+CC and 2444TC+CC in risk of developing gastric cancer.