Relationship Between Thrombospodin-1 Expression And Angiogenesis In Osteosarcoma

OBJECTIVE: To explore the expression of Thrombospodin-1 (TSP-1) in osteosarcoma and investigate its relationship between the expression and invasion. microvessel density of adenomas.METHODS: 54 cases with osteosarcoma tissue specimens were obtained from the pathology department of Lanzhou University First Hospital and Gansu Provincial Tumor Hospital between January 2003 and February 2007, fixed in 4% paraformaldehyde, embedded in paraffin. The expression of TSP-1 was evaluated by immunohistochemical staining of tumor speciments from 54 patients with osteosarcoma. The microvessel density (MVD) was assessed by immunostaining with CD34 in the most neovascularized area(hot spots). Images were read and enlarged by research-level digital camera fluorescence microscope and entered into the computer. The data were entered into SPSS13. 0 to proceed statistical analysis to find out the relationship between angiogenesis and TSP-1 expression in osteosarcoma and their clinicopathological significance.RESULTS: The positive rate of TSP-1 in 54 osteosarcoma specimens was 33.3% ( 18/54 ) . In osteosarcoma, the mean microvessel count of TSP-1 trong-expression tumors was significantly lower than that of TSP-1 low-expression tumors. The close correlation was found between TSP-1 expression and microvessel density. TSP-1 expression and microvessel density was remarkably correlated with lung etastasis in osteosarcoma. In contrast, no association was found between TSP-1 epression and gender, age, size and location of tumor, osteosarcoma classification, as well as microvessel density.CONCLUSION: TSP-1 might play an important role in the evelopment of osteosarcoma , and TSP-1 regulates invasiveness and ngiogenesis . Thus, TSP-1 might be a newtherapy target in osteosarcoma.