| Recent studies suggest that hydrogen sulfide(H2S),like nitric oxide(NO) and carbon monoxide(CO),is the third gaseous signal molecule. The effects of H2S on cardiovascular system mainly include vasorelaxation and cardiac negative inotropism.It can influence many signal transductive pathways through preconditioning or postconditioning and preserving mitochondrial function associated with the myocardial ischemia-reperfusion injury.The myocardialprotective effect of H2S involves in ameliorating calcium overload,reducing oxidative stress,decreasing inflammation,turning down metabolism and oxygen demand.This review summarizes recent progress in the myocardialprotective effect of H2S which can ameliorate myocardial ischemia-reperfusion injury by the above protective mechanisms. Background:Hydrogen sulfide(H2S) is a new gaseous signal molecule.The effects of H2S on cardiovascular system mainly include vasorelaxation and cardiac negative inotropism through opening ATP sensitive potassium channels and inhibiting L-type calcium channels in cardiomyocytes,It has been identified that H2S might be a potent myocardialprotective agent,which provide a novel approach to the treatment of ischemic heart disease such as myocardial infarction.Objective:The purpose of this study mainly was to observe the direct effect of hydrogen sulfide(H2S) with different concentrations on the neonatal rat myocardial cells subjected to hypoxia injury,in order to evaluate the concentrations of hydrogen sulfide having protective effects.Methods:Primary-cultured myocardial cells of neonatal rats were randomly divided into 6 groups:control groups and hypoxia groups.The hypoxia groups were divided into S0 group,S100 group,S200 group,S400 group and S800 group according to the concentration of NaHS that is 0μmol/L,100μmol/L,200μmol/L,400μmol/L and 800μmol/L in the solution of hypoxia,which subjected to hypoxia for 48h.Then the number of survival cells and the activity of lactate dehydrogenase(LDH) were measured.Results:The number of survival cells was increased and the activity of LDH was decreased in S100 group,S200 group and S400 group as compared with S0 group(P＜0.01);while the number of survival cells and the activity of LDH in S800 group as compared with S0 group were no significant differences between them(P＞0.05).Conclusion:NaHS of 100μmol/L to 400μmol/L has the protective effects on myocardial cells suffering hypoxia injury,while NaHS of 800μmol/L can not protect them. Background:The dornor heart acturally undergoes a pathophysiological process of ischemia-reperfusion injury during preservation until transplantation, which is critically important for optimal graft's function and survival. A new gaseous signal molecule—hydrogen sulfide(H2S) may induce internal protective mechanisms by preconditioning or postconditioning to ameliorate myocardial ischemia-reperfusion injury.So it may be a new and prospective agent in the preservation solution.Objective:The purpose of this study was to investigate the direct effect of different concentrations of hydrogen sulfide(H2S) on the neonatal rat myocardial cells subjected to hypoxia injury for different periods of time and the indirect effect in response to injury of reoxygenation,in order to analyze the protective effect of hydrogen sulfide on myocardial cells subjected to hypoxia/reoxygenation injury. Methods:Primary-cultured myocardial cells of neonatal rats were randomly divided into 10 groups:control groups;hypoxia groups;hypoxia/ reoxygenation groups.The control groups contained C1 group that is the normal control of the hypoxia groups and C2 group that is the normal control of the hypoxia/reoxygenation groups.The hypoxia groups were divided into H0 group,H100 group,H200 group and H400 group according to the concentration of NaHS that is 0μmol/L,100μmol/L,200μmol/L and 400μmol/L in the solution of hypoxia,which subjected to hypoxia for 24h, 48h,72h.The hypoxia/reoxygenation groups contained R0 group,R100 group,R200 group and R400 group given the same NaHS treatment,which suffered from hypoxia for 24h,48h,72h and reoxygenation for 2h.Then the number of survival cells and the activity of lactate dehydrogenase(LDH) were measured respectively at each time point.Results:At the same time point of 24h,48h,72h hypoxia,the number of survival cells was increased and the activity of LDH was decreased in H100 group,H200 group and H400 group as compared with H0 group(P＜0.01),and the protective effect of hypoxia H200 group,H400 group were better than H100 group at the time point of 24h hypoxia(P＜0.05～0.01);the number of survival cells suffering from hypoxia/reoxygenation was increased and the leakage of LDH from cells suffering reoxygenation was decreased in R100 group,R200 group and R400 group as compared with R0 group(P＜0.01). Conclusion:NaHS of 100μmol/L to 400μmol/L has the protective effects on myocardial cells suffering hypoxia/reoxygenation injury.NaHS of 200μmol/L to 400μmol/L may offer the better protective effects on myocardial cells subjected to hypoxia injury for 24h. |
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