The Change Of Muscle Tissue Of Spastic Rat With Spinal Cord Injury In Morphology And Function

Objective:To establish a stable spasm model of spinal cord injury, intramuscular injection of botulinum toxin type A was applied in order to observe their morphology, spasticity and function score changes before and after injection. Spasms were observed at different times of the formation of botulinum toxin type A, after their morphology, spasticity and function score changes. To identify the effect of botulinum toxin type A to the morphology of the spasm muscle, and compare the change in the morphology of spasm muscle after the botulinum toxin type A was injected at different stage of spasm. To make it clear given after spasm after botulinum toxin type A change in their morphology, and further formed at different times to give a clear spasm Botulinum toxin A What is different from its morphology. Methods: establish the model of spinal cord injury with adult SD rat, the were divided into 1-week group,3-week group, saline group and normal control group according to the time of spasm formed. Botulinum toxin type A injection or saline injection were applied, observe the change in electron microscopy, functional score and spasticity and compare with the normal control group 1 week and 3 weeks after injection,. In the observed 1 week after injection and 3 weeks of electron microscopy, functional assessment and spastic changes at the same time, the formation of 1 week of cramps and 3 weeks after the injection of botulinum toxin type A groups of the electron microscope, function and spasticity score for comparison. Results:1, compare before and after injections: after the formation of spastic model, electron microscope results indicate that: sarcomere was shortened significantly, muscle wire was in disorder, H-zone shortened, the number of mitochondria glycogen reduced. After the injection of botulinum toxin type A significant change in sarcomere length, myofilament disarray, Z-line drift, H band broadens, and markedly increased mitochondrial swelling, glycogen is also increased in the number.3 weeks after injection, sarcomere length is longer than 1 week after injection. Function score:BBB ratings are part of the improvement, Ashworth score also significantly reduced.2, spasticity formed after 1 week and 3 weeks compared:electron microscopy results showed that:cramps 3 weeks than 1 week shorter sarcomere, Z-line drift is more obvious, H-zone with a significantly reduced compared with 1 week. In the post-injection of botulinum toxin type A has a variable-length sarcomere, mitochondria swelling and the Z-line drift, but the sarcomere length of the 3 weeks group compared with the injection of more 1-week group before the opposite. Function score:the improvement of function after injection of both, BBB ratings are part of the increase, Ashworth score also significantly lower function scores slightly better three weeks than 1 week. Conclusion:1, A-type botulinum toxin can significantly reduce the level of muscle spasm; 2, Spasm lasts longer required the higher amount of toxin 3. part of muscles function improvement has been achieved after injection of botulinum toxin type A;...