Association Of Vitamin D Receptor Gene Polymorphisms With Alcoholic Liver Disease

AIM: To investigate the relationship between vitamin D receptor gene polymorphism and susceptible to alcoholic liver disease (ALD), and expect to provide evidence of molecular biology for the prevention and treatment of alcoholic liver disease.METHODS: The Semi-nested PCR-restriction fragment length polymorphism (semi-nested PCR-RFLP) technology was used to analyze 50 cases of alcoholic liver disease patients and 72 healthy controls of the VDR genotype, genotype frequencies, allele frequencies, and finally carried out a correlation analysis.RESULTS: We found three genotypes in the patients group and controls group, BB, Bb and bb. Through the Hardy-Weinberg equilibrium genetic testing, two groups of allele distribution meet with Hardy-Weinberg genetic balance law. In the case group, BB, Bb and bb genotype frequencies were accounted for 8.0%, 12.0% and 80.0%. B, b allele frequencies were accounted for 14.0%, 86.0%; in the control group, BB, Bb and bb genotype frequencies were accounted for 26.4%, 13.9% and 59.7%. B, b allele frequencies were accounted for 33.3%, 66.7%. The case group compared with the control group, genotype and allele frequencies of the distribution between the case group and the control group was significantly difference, in the case group, bb genotype frequency increased significantly, x~2=7.16, P=0.028, OR =2.698, 95%CI: 1.167-6.235; b allele frequency increased significantly, x~2=11.64, P=0.001, OR =3.071, 95%CI: 1.583-3.958. Through a multi-factor unconditional Logistic regression model, found carrying bb genotype of the individual can easily develop into the ALD, bb genotype may be a kind of genetic risk factor in ALD , OR = 2.272, OR 95% CI: 0.971-5.318. The survey results are statistically significant, P <0.05.CONCLUSON: The vitamin D receptor gene Bsm1 sites polymorphism is strongly associated with the ALD, bb genotype may be a risk factors contributing to the development of ALD.