Experimental Study On Jiaweisinisan On Hepatic Fibrosis

Objective: To study the effect of jiaweisinisan(JWSNS) on the prevention and its mechanism. Accordingly, it can offer preferably theoretics and experimentation thereunder for small animal clinic.Methods: 24 adult healthy hybrid dogs weighed about 10 -15kg were used as experimental animals, and were randomly divided into 3 groups,the model group,the Chinese herb group and the colchicines group. Hepatic fibrosis in dogs was induced by mixture of carbon tetrachloride and colza oil. the dogs of Chinese herb group and colchicines group were given JWSNS and colchicines respectively to observe the effects of JWSNS and colchicines on the formation of hepatic fibrosis. Before and after the test,the blood cell were counted and the serum levels of Total protein(TP),Albumin(ALB),Alanine aminotransferase(ALT), Aspartate aminotransferase(AST),Total bilirubin(TBIL),Direct bilirubin(DBIL),Ma!ondia-ldehyde(MDA),Superoxide dismutase(SOD), glutathione peroxidase(GSH-px), Hydroxyp-roline(HyP) were determined each week.Hydroxyproline(HyP) level in the liver was detected after the experiment.Reslut: The WBC of Chinese herb group had shown a rising trend from the start to the third week, declined in the fourth week ,a rebound increased in the fifth week,then came down in the sixth week. The WBC of Chinese herb group showed significant changes compare with the experiment start during the three,four,five weeks (P<0.05) .but it showed no significant difference compared with the experiment start during the six,seven weeks (P>0.05) . The serai levels of ALB of Chinese herb group showed significant changes compare with that of model group during the seven weeks (P<0.01) .The serm levels of DBIL of Chinese herb group showed no significant changes compared with the experiment start during the seven weeks (P>0.05 ),but that of model group showed significant changes compared with the experiment start during the seven weeks (P<0.05) . the serm levels of ALT of Chinese herb group showed significant difference compared with that of model group from the second week (P<0.05) . the serm levels of AST of Chinese herb group is significantly lower than that of model group from the fourth week (P<0.05) . the serum superoxid dismutase(SOD) and glutathione peroxidase(GSH-px) activity of Chinese herb group were significantly highter than that of model group,and the malondialdehyde(MDA) concentration was significantly lower than that of model group,the serum hydroxyproline concentration in Chinese herb group is significantly higher than that of model group(P<0.05) from the second week. At the end of the trial, the collagen content in liver tissues in Chinese herb group is significantly lower than that of model group (P<0.01) .Conclusion: It's mechanism possibly has something with improve the canine liver function, enhance antioxidation, Inhibit peroxidation of lipid, Inhibit collagen synthesis, Promote collagen degradation and Slow progression of hepatic fibrosis. It has more powerful anti-inflammatory effect than that of colchicines. it is a availability prescription of prevent hepatic fibrosis.