| Flavonoids widely resided in the natural world are compounds which people diet all kinds of substances including per day, and distributed the most plant medicament and have less side-effect and extensive pharmacology effect. Because of its extensive biological activities, their structures, syntheses and pharmacological activities have attracted wide attention in recent years.In this thesis, we have selected the flavonoid as the target, synthesized, characterized a series of flavone-6-carboxylic acid and improved on the technics. Biological activities and structure-activity relationship were approach. Our work was carried out as follows:1. The recent progress of the biological activites, synthesis, struture-activity relationship of the flavone have been reviewed extensively based on the detailed investigation of related literatures.2. Antibacterial activities of these compounds were studied by the method of filter paper. The results indicate that these products had active resistance to Echeric-hia coli,Staphyloccocus aureus rosenbach and Bacillus subtilis, and the antimicrobial activity was related with the spatial struetures of these compounds.The antimicrobial activity effect of four factors, including density, solvent, pH and temperature, were studied. And antimicrobial mechanism was preliminarily investigated.3. Cervical cancer was cultured in vitro. The antiproliferation effect of flavone-6- carboxylic acid on cell morphologic changes and growth were detected by MTT assay, which provided theoretical basis for the further studies on Pharmacological mechanism. MTT assay showed that these six compounds can inhibit the proliferation of cervical cancer Hela cells in a dose-dependent and time-dependent manner.4. The total synthesis route of flavone-6-carboxylic acid was chosen in this paper, and improved on the technics. And each factor was studied. This synthetic route provided a direction for the research of synthesizing chalcone in a green, environmental protection method. |
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