Effect Of GCs Used Alone And Combined With Bortezomib On KM3 Cells In Vitro

ObjectiveTo determine proliferation-inhibiting and cell apoptosis capability of glucocorticoids used alone or in combination with bortezomib on KM3 and MM primary cells in vitro.MethodsKM3 cells were treated with different concentrations of glucocorticoids or combined with bortezomib, and then cell viability were analyzed with MTT assay test. After KM3 cells and MM primary cells were incubated with different concentrations of GCs(Dexamethasone, Methylprednisolone and Hydrocortisone) or GCs in combination with bortezomib, the percentage of earlier apoptosis cell was analyzed by flow cytometry.Results(1) GCs or bortezomib effectively inhibited the proliferation of KM3 cells in a concentration-dependent way, and the IC50 of GCs after 48hrs was 64.06ug/ml,95.01 ug/ml,105.07 ug/ml. Schedules of bortezomib and GCs resulted in additive interactions(2) Flow cytometry detection testify GCs can induce the apoptosis of KM3 and MM primary cells in a concentration-dependent way in vitro. ConclusionsGCs can inhibit proliferation and induce apoptosis on KM3 and CML primary cells effectively on a concentration-dependent manner. Effects of combinations with bortezomib were additive.Among these GCs, hydrocortisone might be the most sensitive to MM cells in vitro according to the principle of equivalent dose conversion of GCs.