The Effect Of Taxol In The Proliferation, Apoptosis And Invasion Of Choriocarcinoma JEG-3 Cells

Objective : To study the influence of Taxol in the proliferation,apoptosis and invasion of human choriocarcinoma IEG-3 cells.Method: The proliferation of choriocarcinoma JEG-3 cells and the influence of Taxol in the proliferation of JEG-3 cells were detected by MTT; the effect of Taxol in the apoptosis and cell cycle of JEG-3 cells was observed by flow cytometry; the influence of Taxol to the invasive ability of JEG-3 cells was tested by transwell.Result: (1) The number of JEG-3 cells increases as time extends. The intergroup differences are all statistically significant (P<0.05) except the one between 72-hour group and 72-hour midcourse changing group (P>0.05).(2) Under the effect of taxol of the same concentration, the suppression rate of the proliferation of JEG-3 cells rises as time extends. The intergroup difference is significant (P<0.05). The suppression rate of the proliferation of JEG-3 cells rises with the increase of the concentration of taxol in the same duration. The intergroup difference is significant (P<0.05). The 72-hour IC50 calculated by Probit regression analysis is 0.1ug/ml, which is basically equal to the IC50 of malignant tumors that are sensitive to the clinical chemotherapy of taxol.(3) Under the effect of taxol of the same concentration, the suppression rate of the apoptosis of JEG-3 cells rises as time extends. The intergroup difference is significant (P<0.05). The suppression rate of the apoptosis of JEG-3 cells rises with the increase of the concentration of taxol in the same duration and the intergroup difference is significant (P<0.05). The G2/M stage cell percentage of JEG-3 cells acted with taxol of different concentration rises compared with control group and the differences are significant (P<0.05).(4) The in vitro invasive ability of JEG-3 cells to the basic membrane component Matrigel decreases obviously 24 hours after the treatment of taxol of difference concentration and the suppression rate of invasion rises. The intergroup difference is significant (P<0.05).Conclusions: 1. Taxol is able to inhibit the proliferation of choriocarcinoma JEG-3 cells and the effect is time and concentration-dependent.2. Taxol is able to induce the apoptosis of JEG-3 cells and the effect is time and dosage-dependent.3. Taxol can suppress the in vitro invasive ability of JEG-3 cells manifestly.